桦褐孔菌多糖的分离纯化及体外抗氧化活性研究

Isolation, purification and antioxidant activity in vitro of polysaccharides from Inonotus obliquus

中文关键词:  桦褐孔菌  多糖  分离纯化  结构鉴定  抗氧化活性

英文关键词:Inonotus obliquus  polysaccharide  isolation and purification  structure identification  antioxidant activity

中文摘要:

      目的 分离纯化桦褐孔菌多糖(Inonotus obliquus polysaccharide, IOP), 分析其结构并对其抗氧化活性进行评价。方法 以桦褐孔菌为原材料, 采用水提醇沉工艺, 经大孔树脂吸附法除杂脱色得到IOP; 通过DEAE-52纤维素柱对IOP进行分离纯化, 得到4个多糖组分: IOP-1、IOP-2、IOP-3和IOP-4, 通过Sephadex G-100凝胶柱纯化得率较高的组分IOP-1, 得到均一多糖IOP-1a。对IOP-1a进行结构鉴定, 并考察其体外抗氧化能力。结果 IOP-1a相对分子质量为12040 Da; 由7种单糖组成: 葡萄糖、半乳糖、木糖、甘露糖、鼠李糖、阿拉伯糖和岩藻糖(0.341:0.246:0.150:0.102:0.842:0.559:0.213), 红外光谱显示IOP-1a含有β型糖苷键、羰基、羟基等官能团。体外抗氧化实验表明, IOP和IOP-1a均有较好的抗氧化能力。结论 从IOP中分离纯化得到的中性组分IOP-1a具有较好的抗氧化活性。

英文摘要:

      Objective To isolate and purify Inonotus obliquus polysaccharide (IOP), and analyze its structure and evaluate its antioxidant activity. Methods Inonotus obliquus was used as raw materials, the IOP was obtained by water extraction and alcohol precipitation by removing impurity and decolorization using macroporous resin adsorption; the IOP was purified by DEAE-52 cellulose column to obtain 4 kinds of polysaccharide fractions: IOP-1, IOP-2, IOP-3 and IOP-4. IOP-1 with higher yield was purified by Sephadex G-100 gel column to obtain homogeneous polysaccharide IOP-1a. The structure of IOP-1a was identified and its antioxidant capacity in vitro was investigated. Results The relative molecular weight of IOP-1a was 12040 Da; it was composed of 7 kinds of monosaccharides: Glucose, galactose, xylose, mannose, rhamnose, arabinose and fucose (0.341:0.246:0.150: 0.102:0.842:0.559:0.213), and Infrared spectroscopy showed that IOP-1a contained β-glycosidic bond, carbonyl, hydroxyl and other functional groups. Antioxidant experiments in vitro showed that both IOP and IOP-1a had good antioxidant capacity. Conclusion The neutral component IOP-1a isolated and purified from Inonotus obliquus polysaccharide has good antioxidant activity.