食品沙门氏菌环介导等温扩增技术应用研究进展

Research progress of loop-mediated isothermal amplification in the detection of Salmonella in food

中文关键词:  环介导等温扩增  沙门氏菌  血清型  多重环介导等温扩增  检测方法  特异性探针  即时检测

英文关键词:loop-mediated isothermal amplification  Salmonella  serotype  multiplex loop-mediated isothermal amplification  detection methods  specific probe  point-of-care testing

中文摘要:

      沙门氏菌是一种食源性致病菌, 可导致胃肠炎、败血症等严重疾病, 引发人员健康和财产损失, 快速、简便的检测方法对于食品中沙门氏菌的检验至关重要。环介导等温扩增(loop-mediated isothermal amplification, LAMP)作为传统聚合酶链反应(polymerase chain reaction, PCR)的有力替代工具, 经历多年发展已在等温扩增领域显示出明显优势, 在沙门氏菌检测应用方面日渐成熟。本文对近5年来LAMP技术在沙门氏菌检测方法及应用进行了分析汇总。在沙门氏菌LAMP检测技术的特异性方面, 对现有靶标基因和新靶标基因的有效性进行了探讨, 对不同血清型的特异性检测和多重LAMP的应用进行了总结, 分析其优点与不足之处, 阐述了探针法、多重检测、微流控技术和即时检测(point-of-care testing, POCT)是沙门氏菌LAMP技术的未来发展方向, 为LAMP技术更好地应用于食品中沙门氏菌检测提供参考依据, 对于提高食品中沙门氏菌的检验能力具有重要意义。

英文摘要:

      Salmonella is a food-borne pathogen that can cause serious diseases such as gastroenteritis and sepsis, and cause loss of health and property. Fast and simple detection methods are essential for the detection of Salmonella in food. As a powerful alternative to polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) has achieved obvious advantages in the field of isothermal amplification after years of development, and has also got significant achievement in the detection of Salmonella. This article analyzed and summarized the progress and the application of LAMP technology in the detection of Salmonella in the past five years. In terms of the specificity of LAMP technology for Salmonella detection, the existing target genes and new target genes were discussed for its effectiveness, the specific detection of different serotypes and the application of multiple LAMP were summarized, and its advantages and disadvantages were analyzed, which mean the probe method, multiple detection, microfluidic technology and point-of-care testing (POCT) are the guidence of Salmonella LAMP technology. It provides a reference for the better application of LAMP technology in the detection of Salmonella, and is of great significance for improving the detection ability of Salmonella in food.