吡唑醚菌酯在枇杷中的残留行为及膳食风险评估

Residue behaviours and risk assessment of pyraclostrobin in loquat

中文关键词:  吡唑醚菌酯  枇杷  残留  膳食风险评估

英文关键词:pyraclostrobin  loquat  residue  dietary risk assessment

中文摘要:

      目的 探究吡唑醚菌酯在枇杷中的残留特性, 评价其使用安全性。方法 样品经乙腈提取, N-丙基乙二胺分散吸附剂净化, 0.1%甲酸水溶液和乙腈作为流动相进行梯度洗脱, 在多反应监测模式下采用超高效液相色谱-串联质谱法检测, 采用基质匹配标准溶液外标法定量。结果 在0.01、0.10、1.00和3.00 mg/kg添加水平下, 吡唑醚菌酯在枇杷样品中的平均添加回收率在92%~96%之间, 相对标准偏差为3.3%~5.4%, 定量限为0.01 mg/kg。吡唑醚菌酯在枇杷中的消解半衰期为9.5~12.8 d。风险评估结果表明, 我国普通人群摄入吡唑醚菌酯的风险概率为60.3%。结论 在30%吡唑醚菌酯悬浮剂、按推荐剂量(有效成分250~375 mg/kg)施药2~3次、施药间隔7 d、推荐安全间隔期为14 d的条件下, 吡唑醚菌酯残留对我国一般人群产生的风险在可接受范围内。

英文摘要:

      Objective To explore the residual characteristics of pyraclostrobin in loquat, and evaluate its safety. Methods The samples were extracted with acetonitril, cleaned-up by primary secondary amine, and separated by mobile system of 0.1% formic acid and acetonitril with gradient elution. The extracts were determined by ultra performance liquid chromatography-tandem mass spectrometry under multiple reaction monitoring mode and quantified by the matrix matching standard solution external standard method. Results The average recoveries varied from 92% to 96% for pyraclostrobin residues with four spiked levels of 0.01, 0.10, 1.00 and 3.00 mg/kg. The relative standard deviations were 3.3%?5.4% and the limits of quantitation were 0.01 mg/kg. The dissipation rate of pyraclostrobin in loquat followed the first-order kinetics with the half-lives of 9.5?12.8 days. The risk probability was calculated as 60.3% for the general population. Conclusion Under the conditions of 30% pyrazole ether ester suspension, 2?3 times at the recommended dose (active ingredient 250?375 mg/kg), 7 d interval and 14 d recommended safety interval, the risk of pyraclostrobin residue to the general population in China is acceptable.