超高效液相色谱-串联质谱法测定功能饮料中的牛磺酸和赖氨酸

Determination of taurine and lysine in functional drinks by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  超高效液相色谱-串联质谱法  牛磺酸  赖氨酸  功能饮料  苯甲氧羰酰琥珀酰亚胺

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  taurine  lysine  functional drinks  benzoxycarbonyl succinimide

中文摘要:

      目的 建立超高效液相色谱-串联质谱法测定功能饮料中牛磺酸和赖氨酸的检测方法。方法 采用衍生化的方法, 将稀释之后的饮料样品中的牛磺酸和赖氨酸与苯甲氧羰酰琥珀酰亚胺在碱性条件下进行反应, 之后采用超高效液相色谱-串联质谱法测定衍生化后的化合物含量。结果 牛磺酸和赖氨酸分别衍生之后的化合物在1~200 ng/mL的范围内线性良好, 牛磺酸的检出限为20 pg/mL, 定量限为50 pg/mL; 赖氨酸的检出限为100 pg/mL, 定量限为250 pg/mL。2种物质在低、中、高3个浓度的加标回收率为92.77%~107.59%, 相对标准偏差均小于10% (n=6)。结论 本方法样品制备步骤简便、检测灵敏度高、重复性好, 可用于快速准确的测定饮料中的牛磺酸和赖氨酸的含量。

英文摘要:

      Objective To establish a method for the determination of taurine and lysine in functional drinks by ultra performance liquid chromatography-tandem mass spectrometry. Methods Taurine and lysine in diluted beverage samples were reacted with benzoxycarbonyl succinimide under alkaline conditions by derivatization method, and then the content of derivatized compounds was determined by ultra performance liquid chromatography-tandem mass spectrometry. Results The compounds derived from taurine and lysine had good linearities in the range of 1?200 ng/mL, the limit of detection of taurine was 20 pg/mL, and its limit of quantification was 50 pg/mL; the limit of detection of lysine was 100 pg/mL, and its limit of quantification was 250 pg/mL. The recoveries of the 2 kinds of substances at low, medium and high concentrations were 92.77%?107.59%, and the relative standard deviations were less than 10% (n=6). Conclusion This method has the advantages of simple sample preparation steps, high detection sensitivity and good repeatability, which can be used for the rapid and accurate determination of taurine and lysine in functional drinks