呋虫胺及其代谢物在稻米中的低温储藏稳定性研究

Study on the storage stability of dinotefuran and its metabolites in rice under low temperature

中文关键词:  呋虫胺及其代谢物  残留  稻米  储藏稳定性

英文关键词:dinotefuran and its metabolites  residues  rice  storage stability

中文摘要:

      目的 采用高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)测定稻米样品中呋虫胺及其代谢物残留, 考察呋虫胺及其代谢物在稻米中的低温储藏稳定性。方法 样品经乙腈提取后, 采用QuEChERS方法净化, 以0.1%甲酸-乙腈混合溶液为流动相梯度洗脱, 多反应监测(multiple reaction monitoring, MRM)扫描模式, 基质匹配标准溶液外标法定量。结果 本方法在0.0005~0.5000 mg/L范围内线性关系良好, 呋虫胺及其2种代谢产物在0.05、0.50、1.00和5.00 mg/kg添加水平的平均回收率为73.8%~103.1%, 相对标准偏差(relative standard deviations, RSDs)为0.53%~7.94% (n=5), 方法定量限为0.05 mg/kg。?18 ℃储藏条件下稻米样品中呋虫胺及其代谢物的平均降解率均未超过30%。结论 该方法能够满足农药残留检测要求, 适用于稻米中呋虫胺及其代谢物残留量分析。?18 ℃条件下呋虫胺及其代谢物在稻米样品中的储藏稳定期至少为180 d。

英文摘要:

      Objective To determine the residues of dinotefuran and its metabolites in rice samples by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and to investigate the storage stability of dinotefuran and its metabolites in rice under low temperature. Methods Rice samples were extracted by acetate, purified by QuEChERS method, gradiently eluted in aqueous solution containing 0.1% formic acid solution and acetate, quantization was performed by using selected multiplereaction monitoring mode, and quantified by external standard method. Results The established method had good linear relationship in the range of 0.0005?0.5000 mg/L, the average recoveries were ranged from 73.8% to 103.1% for the dinotefuran and its metabolites residues with 4 spiked levels of 0.05, 0.50, 1.00 and 5.00 mg/kg, the relative standard deviations (RSDs) were 0.53%?7.94% (n=5), and the limits of quantitation were 0.05 mg/kg. Under the storage condition of ?18 ℃, the average degradation rates of dinotefuran and its metabolites in rice samples were less than 30%. Conclusion This method can meet the requirements of pesticide residues and is suitable for the analysis of dinotefuran and its metabolites residues in rice. Under the condition of ?18 ℃, the storage stability of dinotefuran and its metabolites in rice is at least 180 days.