湿法消解-电感耦合等离子体发射光谱法检测富硒豆类中硒蛋白含量

Determination of selenium protein in Se-enriched beans by wet digestion-inductively coupled plasma optical emission spectrometry

中文关键词:  湿法消解  电感耦合等离子体发射光谱法  富硒豆类  硒蛋白

英文关键词:wet digestion  inductively coupled plasma optical emission spectrometry  Se-enriched beans  selenium protein

中文摘要:

      目的 结合不同提取方式建立湿法消解-电感耦合等离子体发射光谱法(inductively coupled plasma optical emission spectrometry, ICP-OES)检测富硒豆类中硒蛋白含量的方法。方法 分别以水、0.5 mol/L氯化钠溶液、75%乙醇(V:V)、0.1 mol/L氢氧化钠溶液为溶剂, 超声辅助提取富硒豆类中的硒蛋白, 硝酸-过氧化氢体系湿法消解样品, 采用ICP-OES检测硒蛋白含量。结果 以氢氧化钠溶液作提取剂, 硒元素分析谱线为196.0 nm, 检测效果最佳, 线性关系良好, 相关系数大于0.99, 检出限为0.082 mg/kg, 定量限为0.272 mg/kg。硒标准溶液的加标回收率为90.0%~98.5%, 相对标准偏差为3.1%~6.3%, 硒代蛋氨酸标准溶液的加标回收率为84.0%~92.8%, 相对标准偏差为0.2%~1.8%。结论 该方法前处理提取效果好, 湿式消解法消解样品完全,方法的物理化学干扰少, 具有良好的准确度和精密度, 适用于富硒豆类食品中硒蛋白含量的准确检测。

英文摘要:

      Objective To establish a method for the determination of selenium protein in Se-enriched beans using wet digestion-inductively coupled plasma optical emission spectrometry (ICP-OES) combined with different extraction methods was proposed. Methods Four extraction solvents: Water, sodium chloride solution (0.5 mol/L), ethanol (75%, V:V) and sodium hydroxide solution (0.1 mol/L) were used to extract selenium protein in Se-enriched beans assisted by ultrasonic, the samples were digested with nitric acid hydrogen peroxide system by wet digestion method, and the selenium protein were determined by ICP-OES. Results Sodium hydroxide solution was used as extractant and the spectral line of selenium was 196.0 nm, the result was the best, the selenium elements had good linear relationships, and the correlation coefficients were greater than 0.99, the limits of detection were 0.082 mg/kg, the limits of quantification were 0.272 mg/kg. The recoveries of selenium standard solution were 90.0%?98.5%, and the relative standard deviations were 3.1%?6.3%. The recoveries of selenomethionine standard solution were 84.0%?92.8%, and the relative standard deviations were 0.2%?1.8%. Conclusion This method has the best extraction effect, the samples can be completely digested by wet digestion, the method has less physical and chemical interference and good accuracy and precision, which is suitable for the accurate detection of selenium protein in Se-enriched beans.