分散液液微萃取结合气相色谱-质谱法快速测定葡萄酒中20种农药残留

Rapid determination of 20 kinds of pesticide residues in wine by dispersive liquid-liquid microextraction combined with gas chromatography- mass spectrometry

中文关键词:  气相色谱-质谱法  农药残留  分散液液微萃取  葡萄酒

英文关键词:gas chromatography-mass spectrometry  pesticide residues  dispersive liquid-liquid microextraction  wine

中文摘要:

      目的 建立分散液液微萃取结合气相色谱-质谱法(gas chromatography-mass spectrometry, GC-MS)测定葡萄酒中20种农药残留的分析方法。方法 样品经水稀释后, 采用60 μL氯仿和940 μL乙腈进行分散液液微萃取, 3000 r/min离心5 min, 浓缩后采用气相色谱-质谱联用仪检测。结果 在白葡萄酒与红葡萄酒中进行2.5、5.0、10.0 μg/L的添加回收实验, 本方法的平均回收率为66.7%~126.1%, 相对标准偏差为1.3%~27.2%, 方法的检出限为0.025~0.690 μg/L, 定量限为0.082~2.300 μg/L。结论 本方法前处理过程简单、快速、灵敏度极高、定量结果准确, 适用于葡萄酒中多种农药残留的检测。

英文摘要:

      Objective To establish a method for the determination of 20 kinds of pesticide residues in wine by dispersive liquid-liquid microextraction combined with gas chromatography-mass spectrometry (GC-MS). Methods After the samples were diluted with water, 60 μL chloroform and 940 μL acetonitrile were used for dispersion liquid-liquid microextraction, centrifuged at 3000 r/min for 5 min, concentrated and detected by gas chromatography-mass spectrometry. Results The average recoveries of 2.5, 5.0 and 10.0 μg/L in white wine and red wine were 66.7%?126.1%, the relative standard deviations were 1.3%?27.2%, the limits of detection were 0.025?0.690 μg/L, and the limits of quantification were 0.082?2.300 μg/L. Conclusion This method is simple, rapid, highly sensitive and accurate in quantitative results, which is suitable for the determination of various pesticide residues in wine.