游离氨基酸检测方法及其应用

Analysis method and application of free amino acids

中文关键词:  游离氨基酸  分光光度法  离子色谱-积分脉冲安培法  氨基酸分析仪法  高效液相色谱法  液相色谱-串联质谱法

英文关键词:free amino acids  spectrophotometry  ion exchange chromatography integrated pulsed amperometric detection  amino acid analyzer method  high performance liquid chromatography  liquid chromatography-tandem mass spectrometry

中文摘要:

      游离氨基酸是动植物体中重要的活性成分和风味物质, 随着现代科学技术的进步, 游离氨基酸的检测方法多样且应用研究进展迅速, 汇总整理各类检测方法及其相关应用不可或缺。本文综述了常用于检测分析游离氨基酸的分光光度法、离子色谱-积分脉冲安培法、氨基酸分析仪法、高效液相色谱法和液相色谱-串联质谱法5种方法, 以及超临界流体色谱法和近红外光谱法2种新技术, 分析了各检测方法的原理及优缺点, 并对各检测方法列举了相关应用研究。现代新型技术的发展和推广, 多元化离子化方式、高分辨质谱、多级串联质谱等技术将会使游离氨基酸分析的灵敏度、准确度、分析速度及自动化程度提高到一个崭新的水平, 选择性好、准确度高、前处理简便的检测手段将是游离氨基酸检测分析技术的未来发展趋势。

英文摘要:

      Free amino acids are important active components and flavor compounds in plants and animals. With the progress of modern science and technology, the detection methods of free amino acids are diverse and the application research progress is rapid. This paper reviewed 5 kinds of methods, including spectrophotometry, ion exchange chromatography integrated pulsed amperometric detection, amino acid analyzer, high performance liquid chromatography and liquid chromatography-tandem mass spectrometry, and 2 kinds of new technologies (supercritical fluid chromatography and near infrared spectroscopy). Then this paper analyzed the principle, advantages and disadvantages of each detection method, and listed the related application research of each detection method. With the development and popularization of modern new technologies, diversified ionization methods, high resolution mass spectrometry, multi-stage tandem mass spectrometry and other technologies, the sensitivity, accuracy, analysis speed and automation of free amino acid analysis would be improved to a new level. The detection methods with good selectivity, high accuracy and simple pretreatment would be the future development trend of free amino acid detection and analysis technology.