QuEChERS-液相色谱-串联质谱法快速测定保健食品中违禁添加药物托拉塞米和双醋酚丁含量

Rapid determination of illegal additives torasemide and diaotyldiphenolisatinum in health food by QuEChERS-liquid chromatography-tandem mass spectrometry

中文关键词:  QuEChERS  液相色谱-串联质谱法  托拉塞米  双醋酚丁  保健食品

英文关键词:QuEChERS  liquid chromatography-tandem mass spectrometry  torasemide  diaotyldiphenolisatinum  health food

中文摘要:

      目的 建立QuEChERS-液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)快速测定保健食品中违禁添加药物托拉塞米和双醋酚丁的分析方法。方法 样品经乙腈提取后, 用QuEChERS法净化, Symmetry C18色谱柱分离, LC-MS/MS外标法定量。结果 该方法具有较高的灵敏度, 托拉塞米和双醋酚丁的定量限为0.2 μg/kg; 在0.2~50.0 μg/kg浓度范围, 线性相关系数大于等于0.9986, 平均回收率为87.6%~98.9%, 相对标准偏差为3.8%~10.5%。结论 该方法处理过程快速简便, 灵敏度高、专属性强, 适用于保健食品中物托拉塞米和双醋酚丁的含量的测定。

英文摘要:

      Objective To establish a rapid method for the determination of torasemide and diaotyldiphenolisatinum in health food by QuEChERS-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods After extracted by acetonitrile, the samples were purified by QuEChERS method, separated by symmetry C18 column and quantified by LC-MS/MS with external standard method. Results The high sensitivity of this method was confirmed by methodology, the limits of quantification of torasemide and diaotyldiphenolisatinum were 0.2 μg/kg, and the linear correlation coefficients were greater than or equal to 0.9986 respectively in the concentration range of 0.2?50.0 μg/kg, the average recoveries were 87.6% to 98.9%, and the relative standard deviations were 3.8%?10.5%. Conclusion This method is quick and simple, sensitive and specific, and suitable for the determination of tolasemide and diaotyldiphenolisatinum in health food.