气相色谱法-电子捕获检测器同时测定鸡蛋中19种农药

Determination of 19 kinds of pesticide residues in eggs by gas chromatography-electroncapture detector

中文关键词:  有机氯  拟除虫菊酯类  鸡蛋  固相萃取  气相色谱法-电子捕获检测器

英文关键词:organochlorine  pyrethroid  eggs  solid phase extraction  gas chromatography-electroncapture

中文摘要:

      目的 建立气相色谱法测定鸡蛋中19种有机氯及菊酯类农药残留的分析方法。方法 样品经乙腈提取, 对比4种净化方式筛选出最佳方式, 采取固相萃取法和分散固相萃取法(QuEChERS)进行样品净化, 固相萃取柱包括GCB/NH2柱、GCB/PSA柱、FLORISIL柱, 然后经气相色谱法-电子捕获检测器分析, 外标法定量。结果 经过QuEChERS净化后, 基质标准曲线线性关系良好, 相关系数在0.9974~0.9999之间, 回收率在70.3%~109.6%之间, 相对标准偏差在0.17%~7.2%之间, 检出限在0.15~5.1 μg/kg之间, 具有良好的线性关系及精密度。结论 该方法简单、准确、高效, 可用于鸡蛋中有机氯及菊酯类农药残留的快速筛查和定量检测。

英文摘要:

      Objective To establish a method for the determinationof 19 kinds of organochlorine pesticide and pyrethroid residues in eggs samples by gas chromatography. Methods Eggs samples were extracted with acetonitrile, through comparing four kinds of purification methods, the best detection method was selected. The samples were purified by solid phase extraction (SPE) and dispersed solid phase extraction (QuEChERS). The SPE columns included GCB/NH2 column, GCB/PSA column and FLORISIL column. And them were detected by gas chromatography-electroncapture detector (GC-ECD). The external standard method was used for quantification. Results Excellent linearities were achieved for all targetanalytes with good linear correlation and correlation coefficients ranged from 0.9974-0.9999. The recoveries were in the range of 70.3%–109.6%, with relative standard deviations (RSDs) range of 0.17%–7.2%, the limits of detection (LODs) were ranged from 0.15 to 5.1 μg/kg. Conclusion In summary, the proposed method is simple, accurate, efficient, and is adequate for the rapid screening and quantitative analysis of organochlorine pesticideand pyrethroid residues in eggs.