食品中金黄色葡萄球菌测量不确定度评定

Evaluation of uncertainty in measurement of Staphylococcus aureus in food

中文关键词:  金黄色葡萄球菌  测量不确定度  食品安全

英文关键词:Staphylococcus aureus  measurement uncertainty  food safety

中文摘要:

      目的 建立食品中金黄色葡萄球菌测量不确定度的评定方法案列, 为食品微生物定量检测提供科学理论指导。方法 依据GB 4789.10—2016《食品安全国家标准 食品微生物学检验 金黄色葡萄球菌检验》第二法和JJF 1059.1—2012《测量不确定度评定与表示》, 以人工污染金黄色葡萄球菌的奶粉样品为例, 分析样品制备、稀释过程、加样过程及重复性检测引入的不确定度, 构建数学模型评定不确定度。结果 样品中金黄色葡萄球菌检测结果的扩展不确定度U=0.490(k=2)。结论 金黄色葡萄球菌测定过程中的不确定度主要由单次检测引入的不确定度和重复测量的不确定度组成, 样品重复性检测引入的不确定度贡献最大。

英文摘要:

      Objective To establish a measurement method for evaluating the uncertainty of Staphylococcus aureus in food, providing a scientific theoretical guidance for quantitative detection of microorganisms for food. Methods Based on the second method of GB 4789.10—2016 National food safety standard-Food microbiological examination-Staphylococcus aureus test and JJF 1059.1—2012 Evaluation and representation of uncertainty measurement, taking a milk powder sample artificially contaminated with Staphylococcus aureus as an example, the uncertainties introduced in sample preparation, dilution process, sample addition process and repeatability test were analyzed, and a mathematical model was constructed to evaluate the uncertainty. Results The expanded uncertainty of detection results of Staphylococcus aureus in samples was U=0.490 (k=2). Conclusion The uncertainty in the determination of Staphylococcus aureus is mainly composed of the uncertainty introduced by a single test and the uncertainty of repeated measurements, and the uncertainty introduced by the repeatability of the sample test contributed the most.