胶体金免疫层析法测定牛羊肉中掺杂鸡肉

Determination of chickenadulteration in beef and mutton by colloidal gold immunochromatography

中文关键词:  牛羊肉  鸡肉  掺杂  胶体金免疫层析

英文关键词:beef and mutton  chicken  adulteration  colloidal gold immunochromatography

中文摘要:

      目的 建立胶体金免疫层析法快速检测牛羊肉中掺杂鸡肉的方法。方法 采用柠檬酸三钠制备胶体金溶液, 以真空干燥的方式制备有金标抗体的微孔试剂, 将鸡IgY包被于硝酸纤维素膜(NC膜)的检测线(T线), 兔抗羊抗体包被于硝酸纤维素膜(NC膜)的质控线(C线), 向硝酸纤维素膜依次贴上吸水纸和样品垫, 切条组装成试纸条。样品经磷酸盐处理, 加入至金标抗体的微孔中, 用试纸条检测, 胶体金分析仪检测T/C线的颜色信号。结果 该方法对牛羊肉中掺入鸡肉的检出限为3%, 重复性和特异性较好, 其他动物组织对其无干扰, 检测时间仅需要15 min。结论 该方法操作简便, 具有较高的灵敏度和特异性, 适用于牛羊肉中掺杂鸡肉的现场快速筛查和检测。

英文摘要:

      Objective To establish a method for the rapid detection of adulterated chicken in beef and mutton by colloidal gold immunochromatography. Methods Colloidal gold solution was prepared by trisodium citrate, and microporous reagent with gold labeled antibody was prepared by vacuum drying. Chicken IgY was conjugated on the detection line (T) of nitrocellulose (NC) membrane, and rabbit anti sheep antibody was conjugated on the quality control line (C) of nitrocellulose (NC) membrane. Absorbent paper and sample pad were attached to nitrocellulose membrane in turn, and then cut and assembled into test strips.The sample was treated with phosphate, added into the microspores of gold labeled antibody, then detected by test strips. The color signal of T/C line was detected by colloidal gold analyzer. Results The limitof detection of this method was 3%, with good repeatability and specificity.The other animal tissues did not interfere with it, and the detection time was only 15 min. Conclusion The method is simple, sensitive and specific, and can be used for on-site rapid screening and detection of chicken adulteration in beef and mutton.