史晓梅,赵文君,李 一,陈海军,张双虹,王 健,王 宝,赵抒娜,谢云峰.液相色谱-串联质谱法测定黑糖中丙烯酰胺和天冬酰胺[J].食品安全质量检测学报,2020,11(24):9266-9272
液相色谱-串联质谱法测定黑糖中丙烯酰胺和天冬酰胺
Determination of acrylamide and L-asparagine in black sugar products by liquid chromatography-tandem mass spectrometry
投稿时间:2020-10-26  修订日期:2020-12-07
DOI:
中文关键词:  液相色谱-串联质谱法  丙烯酰胺  天冬酰胺  黑糖
英文关键词:liquid chromatography-tandem mass spectrometry  acrylamide, L-asparagine  black sugar products
基金项目:
作者单位
史晓梅 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室 
赵文君 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室 
李 一 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室 
陈海军 中粮屯河糖业股份有限公司, 农业部糖料与番茄质量安全控制重点实验室 
张双虹 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室 
王 健 中粮屯河糖业股份有限公司, 农业部糖料与番茄质量安全控制重点实验室 
王 宝 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室;中粮屯河糖业股份有限公司, 农业部糖料与番茄质量安全控制重点实验室 
赵抒娜 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室;中粮屯河糖业股份有限公司, 农业部糖料与番茄质量安全控制重点实验室 
谢云峰 中粮营养健康研究院有限公司, 老年营养食品研究北京市工程实验室, 营养健康与食品安全北京市重点实验室 
AuthorInstitution
SHI Xiao-Mei COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety 
ZHAO Wen-Jun COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety 
LI Yi COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety 
CHEN Hai-Jun COFCO Sugar Co., Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC 
ZHANG Shuang-Hong COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety 
WANG Jian COFCO Sugar Co., Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC 
WANG Bao COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety;COFCO Sugar Co., Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC 
ZHAO Shu-Na COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety;COFCO Sugar Co., Ltd., Key Laboratory of Quality & Safety Control for Sugar Crops and Tomato, Ministry of Agriculture of the PRC 
XIE Yun-Feng COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Engineering Laboratory of Geriatric Nutrition& Foods, Beijing Key Laboratory of Nutrition & Health and Food Safety 
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中文摘要:
      目的 建立同时检测黑糖产品中丙烯酰胺和天冬酰胺的液相色谱-串联质谱法(liquid chromat ography-tandem mass spectrometry, LC-MS/MS)。方法 黑糖产品样品经纯水提取净化后直接测定, 采用含有5 mmol/L的乙酸铵、0.1%的甲酸水(A)和乙腈(B)作为流动相进行梯度洗脱, 质谱采用多反应监测模式监测定性和定量离子。结果 丙烯酰胺和天冬酰胺在0.1、0.2、0.5 mg/L 3个添加浓度上回收率范围为93.5%~108.0%, 相对标准偏差为1.4%~5.4%, 检出限分别为0.01、0.005 mg/L。结论 本方法前处理操作快速简单, 可重复性好, 满足国内外对黑糖产品中丙烯酰胺和天冬酰胺的快速、准确的检测要求, 适合大量样品的准确定量和定性分析。
英文摘要:
      Objective To establish a method for the determination of acrylamide and L-asparagine in black sugar products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods The black sugar product samples were extracted and purified by pure water and directly determined. The mobile phase was gradient elution with 5 mmol/L ammonium acetate, 0.1% formic acid water (A) and acetonitrile (B). The qualitative and quantitative ions were monitored by mass spectrometry using multiresponse monitoring model. Results The recoveries of acrylamide and L-asparagine at 0.1, 0.2, 0.5 mg/L were 93.5%?108.0%, and the relative standard deviations were 1.4%?5.4%. The limits of detection were 0.01, 0.005 mg/L Conclusion This method is simple and fast, and has good repeatability. It can meet the requirements of rapid and accurate detection of acrylamide and L-asparagine in black sugar products at home and abroad, and is suitable for accurate quantitative and qualitative analysis of a large number of samples.
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