实时荧光定量PCR法鉴别俄罗斯鲟鱼子酱分子

Identification of Acipenser gueldenstaedtii caviar molecules by real-time fluorescence quantitative PCR

中文关键词:  俄罗斯鲟  鱼子酱  实时荧光定量PCR  源性成分

英文关键词:Acipenser gueldenstaedtii  real-time fluorescent quantitative PCR  caviar  source compounds

中文摘要:

      目的 建立鉴别荧光定量PCR法鉴别俄罗斯鲟鱼子酱的方法。方法 以鲟形目16种鱼类D-LOOP基因序列作为目的基因进行比对, 选择差异序列设计俄罗斯鲟特异性引物, 构建俄罗斯鲟源性成分实时荧光PCR检测方法。结果 通过特异性检测同目7种近源物种、46种非近源物种, 表明该方法对于俄罗斯鲟源性成分具有较好的特异性; 进行梯度稀释灵敏度检测, 可检出0.1 ng/μL俄罗斯鲟DNA。通过对市售6种鱼子酱共35个样本进行检测, 可检测出鱼子酱中的俄罗斯鲟成分。结论 该荧光检测方法特异性强、灵敏度较高, 适用于鱼子酱中俄罗斯鲟源性成分的鉴定检测。

英文摘要:

      Objective To establish a method for the identification of Acipenser gueldenstaedtii by fluorescence quantitative PCR. Methods The D-Loop gene sequences of 16 species of Acipenser were compared as the target genes. The specific primers were designed to construct a real-time fluorescent PCR method for the detection of Acipenser gueldenstaedtii derived components. Results Through the specific detection of seven species of proximal origin and 46 species of non-proximal origin in the same order, it showed that this method had good specificity for the composition of Acipenser gueldenstaedtii. Gradient dilution sensitivity detection was performed to detect 0.1 ng/L DNA of Acipenser gueldenstaedtii. Through the detection of six kinds of caviar sold in the market (35 samples), the fluorescence method could detect the Acipenser gueldenstaedtii in caviar. Conclusion The fluorescence detection method is specific and sensitive, and it is suitable for the identification and detection of Acipenser gueldenstaedtii.