大菱鲆鱼皮抗氧化胶原肽的制备及特性分析

Preparation and characteristic analysis of antioxidant collagen peptides from turbot skins

中文关键词:  大菱鲆  胶原肽  酶解工艺  抗氧化

英文关键词:turbot  collagen peptides  enzymolysis process  antioxidant activity

中文摘要:

      目的 以大菱鲆(Scophthalmus maximus)鱼皮为原料制备抗氧化胶原肽并分析其特性。方法 在单因素实验基础上通过响应面法优化鱼皮酶解工艺, 并对所制备胶原肽进行G-15葡聚糖凝胶层析分离及体外抗氧化活性检测。结果 大菱鲆鱼皮酶解的最适酶为中性蛋白酶, 最佳工艺条件为: 酶解温度52 °C, 酶解时间为338 min, 加酶量为1142 U/g, 反应体系pH值为6.7。按照此最佳条件制备得到鱼皮酶解液羟自由基清除率为34.44%, 与理论值接近。大菱鲆鱼皮胶原肽经过层析分离, 得到了3种不同分子质量的多肽组分TSP1、TSP2、TSP3, 其中TSP3体外抗氧化活性最好, 具有更强的羟自由基清除能力、亚铁离子螯合能力、苯代苦味肼基自由基清除能力以及还原力。结论 本研究为大菱鲆及其他鱼皮多肽的开发研究以及鱼类副产物高值化利用提供了技术参考。

英文摘要:

      Objective To prepare antioxidant collagen peptides from turbot fish skins and analyze the characteristic. Methods Based on the single factor experiments, the enzymatic hydrolysis conditions of fish skins were optimized by response surface methodology, and the prepared collagen peptides were separated by G-15 dextran gel chromatography and tested for in vitro antioxidant activity. Results The results showed that the most suitable enzyme in the enzymatic hydrolysis process was neutral protease. The optimum technological conditions were determined as follows: the enzymatic hydrolysis temperature was 52 °C, the enzymatic hydrolysis time was 338 min, the amount of enzyme added was 1142 U/g, and the pH of the reaction system was 6.7. According to the optimum conditions, the free radical scavenging rate of turbot skin hydrolysate was 34.44%, which was closed to the theoretical value. After chromatography separation, antioxidant component TSP1, TSP2, TSP3 were finally obtained. The best antioxidant component among them was TSP3, which had strong hydroxyl radical scavenging ability, ferrous ion chelating ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and reducing power. Conclusion This study provides technical reference for the exploitation and research of turbot and other fish polypeptides and the high value utilization of fish by-products.