自动顶空-气相色谱-串联质谱法测定生乳中8种苯系物的研究

Determination of eight benzene series in raw milk by automatic headspace gas chromatography mass spectrometry

中文关键词:  生乳  苯系物  顶空  气相色谱-串联质谱法

英文关键词:raw milk  benzene series  headspace  gas chromatography mass spectrometry

中文摘要:

      目的 建立同时测定生乳中8种苯系物的自动顶空-气相色谱-串联质谱法。方法 试样中的苯系物经顶空进样器加热挥发后进入HP-InnoWax (30 m×0.25 mm, 0.25 μm)毛细管色谱柱进行分离, 采用气相色谱质谱联用法进行测定, 采用外标法定量。结果 8种苯系物在0~25.6 ng/mL浓度范围内线性良好, 相关系数大于0.99, 定量限为0.1~0.8 μg/kg、回收率为85.0%~106.8%、精密度均小于7%。结论 本方法前处理简单、灵敏度高, 可以满足生乳中8种苯系物的同时测定要求。

英文摘要:

      Objective To establish a method for the simultaneous determination of eight benzene series in raw milk by automatic headspace gas chromatography mass spectrometry. Methods The benzene series in the sample were heated and volatilized by the headspace sampler and then entered the HP-InnoWax (30 m×0.25 mm, 0.25 μm) capillary column for separation. The determination was carried out by gas chromatography-mass spectrometry, external standard method was adopted for quantitative analysis. Results The benzene series had good linearity in the concentration range of 0?25.6 ng/mL, the correlation coefficient was greater than 0.99, the limit of quantification were 0.1?0.8 μg/kg, the recoveries were 85.0%?106.8%, and the precision was less than 7%. Conclusion The method has simple pretreatment and high sensitivity, and can meet the requirements of simultaneous determination of eight benzene series in raw milk.