离子色谱-脉冲安培法测定饮料中的聚葡萄糖的含量

Determination of polydextrose in beverage by ion chromatography coupled with pulsed amperometric detection

中文关键词:  聚葡萄糖  饮料  离心超滤器  离子色谱脉冲安培检测

英文关键词:polydextrose  beverage  centrifugal ultrafilter  ion chromatography with pulsed amperometric detection

中文摘要:

      目的 优化离子色谱-脉冲安培法测定饮料中的聚葡萄糖的含量。方法 样品经离心超滤净化, 去除基质干扰, 采用色谱柱分离, 离子色谱-脉冲安培检测器测定, 外标法定量。结果 聚葡萄糖在0.20~2.00 g/L的浓度范围内呈良好线性关系(r2>0.999)。方法检出限和定量限分别为0.01 和0.03 g/L, 3个添加水平的平均加标回收率为90.0%~98.8%, 相对标准偏差为0.63%~2.36%。同时考察了市场上10种不同品类的饮料, 加标回收率为93.0%~105%。结论 本方法样品前处理简单、快速、广泛适用于饮料中添加的聚葡萄糖含量测定。

英文摘要:

      Objective To optimize the ion chromatography coupled with pulsed amperometric detection method for the determination of polydextrose in beverage. Methods The sample was purified with centrifugal ultrafilter to remove interferences. The analyte was separated and detected by ion chromatography with pulsed amperometric detection. Results Under the optimized sample pretreatment conditions, the method showed a good linearity in the concentration range of 0.20?2.00 g/L (r2 >0.999). The detection and quantitation limits were 0.01 and 0.03 g/L, respectively. The average method recoveries were 90.0%?98.8% and relative standard deviation ranged from 0.63% to 2.36%, for the 3 spiking levels. Meanwhile, 10 commercial products in different beverage categories were tested, and the recoveries ranged from 93.0% to 105%. Conclusion This method is simple, rapid, accurate and suitable for the determination of fortified polydextrose in beverages.