高效液相色谱法测定保健食品中磷脂酰丝氨酸含量

Determination of phosphatidylserine in health food by high performance liquid chromatography

中文关键词:  高效液相色谱法  保健食品  磷脂酰丝氨酸

英文关键词:high performance liquid chromatography  health food  phosphatidylserine

中文摘要:

      目的 建立高效液相色谱法测定磷脂酰丝氨酸含量的分析方法。方法 样品经过三氯甲烷溶解, 以正己烷:异丙醇:1%磷酸水溶液=(25:70:5, V:V:V)为流动相, 等度洗脱, 经SIL色谱柱(4.6 mm×250 mm, 5 μm)分离, 并于205 nm波长下检测。结果 磷脂酰丝氨酸在浓度0.13504~0.67520 mg/mL之间呈现良好的线性关系, r2=0.9993, 平均回收率为96.16%~99.60%, 相对标准偏差为1.1%。结论 该方法测定保健食品中磷脂酰丝氨酸含量具专属性强, 耗时短、操作简便、准确、重现性好, 可在实验室推广。

英文摘要:

      Objective To establish a method for determination of phosphatidylserine in health food by high performance liquid chromatography (HPLC). Methods Samples were dissolved by chloroform and separated by SIL chromatographic column (4.6 mm×250 mm, 5 μm), with the mobile phase consisting of n-hexane: isopropanol: 1%phosphoric acid water (25:70:5, V:V:V) by isocratic elution. The detection wave length was 205 nm. Results Phosphatidylserine had a good linear relationship in range of 0.13504~0.67520 mg/mL (r2=0.9993), the average recovery was 96.16%~99.60%, and the relative standard deviation was 1.1%. Conclusion This method has the advantages of high specificity, short time, simple operation, accuracy and good reproducibility in the determination of phosphatidylserine in health food, which can be popularized in the laboratories.