液相色谱质谱联用法测定牛奶中氯霉素的不确定度

Uncertainty evaluation of determination of chloramphenicol in milk by liquid chromatography-tandem mass spectrometry

中文关键词:  液相色谱-串联质谱法  氯霉素  不确定度

英文关键词:liquid chromatography-tandem mass spectrometry  chloramphenicol  uncertainty

中文摘要:

      目的 评定液相色谱质谱联用法测定牛奶中氯霉素的不确定度。方法 根据GB/T 20756-2006《可食动物肌肉、肝脏和水产品中氯霉素、甲砜霉素和氟苯尼考残留量的测定》, 采用液相色谱-串联质谱法测定牛奶中氯霉素残留量, 分析不确定度的来源并对其进行量化。结果 样品中氯霉素含量为5.1837 μg/kg, 取k=2, 得到扩展不确定度为0.67938 μg/kg, 其结果可表示为(5.1837±0.67938) μg/kg。结论 影响检测结果不确定度的主要因素是标准储备液以及标准曲线各点的配制。

英文摘要:

      Objective To evaluate the uncertainty of determination of chloramphenicol in milk by liquid chromatography-tandem mass spectrometry. Methods According to GB/T 20756-2006 Determination of muli-residues of chloramphenicols in edible animal tissue, liver and aquatic products, the determination of chloramphenicol residues in milk was carried out by liquid chromatography-tandem mass spectrometry, sources of uncertainty were analyzed and quantified. Results The content of chloramphenicol in the sample was 5.1837 μg/kg, and k=2. The extended uncertainty was 0.67938 μg/kg, and the result could be expressed as (5.1837±0.67938) μg/kg. Conclusion The main factors affecting the uncertainty of the test results are the preparation of the standard reserve liquid and the points of the standard curve.