两种副溶血性弧菌检测方法的比对

Comparison of 2 detection methods for Vibrio parahaemolyticus

中文关键词:  副溶血性弧菌  方法比对  国家标准  快速检测

英文关键词:Vibrio parahaemolyticus  comparison of methods  national standards  rapid detection

中文摘要:

      目的 比较国家标准中的定性检测法与快速检测法中的普通PCR法在检测食源性副溶血性弧菌上的区别与优劣, 为今后针对副溶血性弧菌的检测方法的改进依据及应对突发食品安全事件时快速检测提供参考。 方法 采用GB 4789.7-2013《食品安全国家标准 食品微生物学检验 副溶血性弧菌检验》与SN/T 1869-2007《食品中多种致病菌快速检测方法 PCR法》对经阳性菌株污染的市售墨鱼干样品进行副溶血性弧菌的检测, 并对2种方法进行对比。结果 2种方法在针对食源性副溶血性弧菌的检验上的比对结果均为检出。在实验耗时方面, 国家标准法确认检出阳性需耗时4 d, 快速检测法仅需耗时26 h。结论 国家标准中的定性检测法与快速检测方法均能检出副溶血性弧菌, 国标法在应对日常检验上标准且能够广泛应用, 快速检测方法在快速、准确地应对食品安全突发事件方面具有优势。

英文摘要:

      Objective To compare the differences, advantages and disadvantages of ordinary PCR methods between the qualitative and rapid detection methodin the national standard for the detection of foodborne Vibrio parahaemolyticus, in order to provide a reference for the future improvement of the detection method for Vibrio parahaemolyticus and rapid detection in response to food safety emergencies. Methods GB 4789.7-2013 National foods safety standard-Food microbiological examination-Vibrio parahaemolyticus test and SN/T 1869-2007 Rapid detection methods for pathogens in foods-PCR method were used for the detection of Vibrio parahaemolyticus and the 2 methods were compared. Results The comparative results of 2 methods on the test for Vibrio parahaemolyticus of food origin were both detectable. In terms of the experimental time, it took 4 d for the national standard method to confirm the positive detection, and only 26 h for the rapid detection method. Conclusion Vibrio parahaemolyticus can be detected by both qualitative and rapid tests in the national standards. National standard method is standard and can be widely used for routine testing. Rapid detection method has advantages in responding quickly and accurately to food safety emergencies.