超高效液相色谱-三重四极杆串联质谱法同时测定劲酒中8种活性成分含量

Simultaneous determination of 8 kinds of active ingredients in Chinese Jing liqueur by ultra performance liquid chromatography tandem mass spectrometry

中文关键词:  中国劲酒  超高效液相色谱-串联质谱法  活性成分

英文关键词:Chinese Jing liqueur  ultra performance liquid chromatography tandem mass spectrometry  active ingredients

中文摘要:

      目的 建立超高效液相色谱-三重四级杆串联质谱(ultra performance liquid chromatography tandem mass spectrometry, UPLC-MS/MS)法同时测定中国劲酒中8种活性成分含量的分析方法。方法 采用Agilent EC-C18色谱柱(4.6 mm×50 mm, 1.8 μm), 以0.1%甲酸水?0.1%甲酸甲醇为流动相, 梯度洗脱, 流速0.2 mL/min。采用电喷雾离子源进行正离子模式监测, 多反应监测模式(multiple reaction monitoring, MRM)用于定量分析。结果 中国劲酒中的8种活性成分在考察的浓度范围内具有良好的线性关系(r>0.9981); 回收率(n=6)在96.7%~103.3%内, 相对标准偏差(relative standard deviation, RSD)均小于4.38%; 3批次间8个成分的含量差异较大。结论 该方法简便快速、专属性强、灵敏度高, 可用于中国劲酒的质量控制。

英文摘要:

      Objective To establish a method for simultaneous determination of 8 kinds of active ingredients in Chinese Jing liqueur by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Methods The chromatographic separations were obtained on a Agilent EC-C18 (4.6 mm×50 mm, 1.8 μm) with a gradient elution using the mixture of 0.1% formic acid methanol and 0.1% formic acid water solution as mobile phase. The flow rate was 0.2 mL/min. The multiple-reaction monitoring (MRM) scanning was employed for the quantification with switching electrospray ion source polarity in positive mode. Results All calibration curves of 8 components were linear (r>0.9981) over the tested ranges. The recoveries (n=6) ranged from 96.7% to 103.3%, and the RSD was less than 4.38%. Among 3 batches, the content of 8 components varied greatly. Conclusions This method is convenient, rapid, and it has good selectivity and high sensitivity, which can be used for the quality control of Chinese Jing liqueur.