高效液相色谱法测定罗汉参中的西瑞香素

Determination of daphnoretin content in Luohanshen by high performance liquid chromatography

中文关键词:  罗汉参  西瑞香素  高效液相色谱法

英文关键词:Luohanshen  daphnoretin  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定罗汉参中西瑞香素含量的分析方法。方法 样品经过乙醇溶液提取, 超声30 min, 水浴温度70 ℃, 旋蒸定容。采用C18反向色谱柱(250 mm×4.6 mm, 5 μm)分离, 以甲醇-0.2%磷酸水溶液(56:44, V:V)为流动相等度洗脱, 流速为1.0 mL/min, 柱温为35 ℃, 检测波长345 nm。结果 西瑞香素浓度在1~20 μg/mL内与色谱峰面积呈良好的线性关系, Y=1.2872X+0.0921, r2=0.9998。方法精密度为0.34% (n=6), 样品中加标回收率的范围从99.9%~101.2%。测定结果的相对标准差为1.73% (n=6), 检出限(S/N=3)为0.06 μg/g。结论 该方法灵敏度高, 可快速准确对罗汉参中的西瑞香素进行检测。

英文摘要:

      Objective To establish a method for the determination of daphnoretin in Luohanshen by high performance liquid chromatography. Methods The sample was extracted by ethanol solution, ultrasonic for 30 minutes, water bath temperature of 70 ℃, spin evaporation and constant volume. The separation was performed on reverse C18 column (250 mm×4.6 mm, 5 μm) with methanol-0.2% phosphoric acid aqueous solution (55:44, V:V) mobile phase. The flow rate was 1.0 mL/min. The column temperature was 35 ℃ and the detection wavelength was 345 nm. Results There was a good linear relationship between the peak area and the concentration of daphnoretin in the range of 1-20 μg/mL, Y=1.2872X+0.0921, r2=0.9998. The precision of method was 0.34% (n=6). The recoveries of samples were from 99.9% to 101.2%. The relative standard deviation was 1.73% (n=6), and the limit of detection was 0.06 μg/g (S/N=3). Conclusion This method has high sensitivity and can be used for the rapid and accurate determination of daphnoretin in Luohanshen.