气相色谱法测定蔬菜中敌百虫的含量

Determination of trichlorfon in vegetables by gas chromatography

中文关键词:  敌百虫  温度  热分解  基质效应

英文关键词:trichlorfon  temperature  thermal decomposition  matrix effect

中文摘要:

      目的 建立气相色谱法测定蔬菜中敌百虫的含量。方法 研究不同色谱条件及消除基质效应对敌百虫测定的影响, 并取蔬菜样品(以青瓜为代表)10 g进行前处理提取后, 用经优化后的色谱条件对敌百虫进行测定, 外标法定量。结果 在进样口温度设为150 ℃, 使用内径0.32 mm的色谱柱以及设置3.0 mL/min的色谱柱流量时, 敌百虫响应较好; 在0.06~2.0 mg/kg的加标水平下, 敌百虫回收率在90.2%~99.8%之间, 相对标准偏差≤4.3%。结论 通过对敌百虫气相色谱条件的优化以及使用基质标准曲线消除基质效应, 可以大幅提高气相色谱法检测敌百虫的准确度。

英文摘要:

      Objective To establish the gas chromatography method to determine the content of trichlorfon in vegetables. Methods The influence of different chromatographic conditions and elimination of matrix effect on the determination of trichlorfon was studied. After taking 10 g of vegetable samples (represented by cucumber) as pretreatment and extraction, the trichlorfon were determined by optimized chromatographic conditions, and quantified by the external standard method. Results When the injector temperature was set to 150 ℃, a chromatographic column with an inner diameter of 0.32 mm was used and a flow rate of 3.0 mL/min was set, the response of trichlorfon was better. At the standard level of 0.06–2.0 mg/kg, the recovery rate of trichlorfon ranged from 90.2% to 99.8%, with the relative standard deviation ≤4.3%. Conclusion By optimizing the gas chromatography conditions of trichlorfon and using matrix standard curves to eliminate matrix effects, the accuracy of gas chromatography detection of trichlorfon can be greatly improved.