芜菁精制多糖的提取、鉴别及含量测定

Extraction, identification and content determination of Brassica rapa L. refined polysaccharides

中文关键词:  芜菁  精制多糖  制备  鉴别  含量测定

英文关键词:Brassica rapa L.  refined polysaccharides  extraction  identification  content determination

中文摘要:

      目的 提取芜菁精制多糖并且对分离得到的精制多糖进行鉴别与含量测定。方法 采用水提醇沉法提取粗多糖后, 采用DEAE-650纤维素柱对芜菁粗多糖洗脱, 从而得到芜菁精制多糖; 对分离得到的精制多糖进行理化鉴别; 采用紫外-可见分光光度法测定芜菁精制多糖含量。结果 分离得到的精制多糖由Molish反应的结果可知组分为糖类物质; 由斐林反应结果可知多糖组分中含具有还原性的基团; 由三氯化铁反应结果可知多糖组分中不含酚类化合物, 且醋酐-浓硫酸反应结果可知不含皂苷类化合物; 精制多糖的平均含量为 581 mg/g。结论 该提取工艺经济, 简单, 稳定, 可作为芜菁精制多糖的提取分离方法, 而且得到的精制多糖含量高, 为后续进一步利用提供基础。

英文摘要:

      Objective To extract the refined polysaccharides from Brassica rapa L., identify and determine the content of the separated refined polysaccharides. Methods After the crude polysaccharide was extracted by water extraction and alcohol precipitation, DEAE-650 cellulose column was used to separate the refined polysaccharide and the physical and chemical identification of the refined polysaccharide was carried out. The content of polysaccharides from turnips was determined by ultraviolet-visible spectrophotometry. Results The refined polysaccharide obtained from the separation could be divided into saccharides according to the Molish reaction results; the reducing group was found in the polysaccharide according to the Fehling reaction results; the phenol compounds were not found in the polysaccharide according to the ferric chloride reaction results, and the results of acetic anhydride-concentrated sulfuric acid reaction showed there were no saponins. The average content of refined polysaccharides was 581 mg/g. Conclusion This extraction process is economical, simple and stable, and can be used as a method to extract and separate the refined polysaccharide from Brassica rapa L..