高效液相色谱串联质谱法测定水产品中的孔雀石绿和结晶紫

Determination of malachite green and crystal violet in aquatic products by high performance liquid chromatography tandem mass spectrometry

中文关键词:  水产品  高效液相色谱-串联质谱法  孔雀石绿  结晶紫

英文关键词:aquatic products  high-performance liquid chromatography-tandem mass spectrometry  malachite green  crystal violet

中文摘要:

      目的 建立高效液相色谱串联质谱法检测水产品中孔雀石绿、隐色孔雀石绿、结晶紫、隐色结晶紫残留量的方法。方法 待检水产品经乙腈提取, 中性氧化铝固相萃取柱净化, 0.22 μm微孔滤膜过滤后用高效液相色谱串联质谱进行检测。结果 4种化合物在0~100.0 ng/mL浓度范围内线性关系良好, 相关系数大于0.999, 检出限均可达到0.25 μg/kg, 回收率为85%~110%, 相对标准偏差均小于10%。结论 本方法使用试剂少、步骤简单, 适用于大批量水产品中孔雀石绿和结晶紫残留量的检测。

英文摘要:

      Objective To establish a method for the determination of residual malachite green, Leucomalachite green, crystal violet and cryptocrystalline violet in aquatic products by high performance liquid chromatography tandem mass spectrometry. Methods The aquatic products was extracted by acetonitrile, purified by neutral alumina solid phase extraction column, filtered by 0.22 μm microporous membrane and detected by high performance liquid chromatography tandem mass spectrometry. Results The linear relationship of the four compounds was good in the concentration range of 0-100.0 ng/mL, and the correlation coefficient was greater than 0.999. The limits of detection were 0.25 μg/kg, the recoveries were 85%-110%, and the relative standard deviation was less than 10%. Conclusion This method uses few reagents and simple steps, and is suitable for the detection of malachite green and crystal violet residues in large quantities of aquatic products.