超高效液相色谱串联质谱法同时测定保健食品中的66种非法添加物

Simultaneous determination of 66 illegal additives in health-care food by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  保健食品  非法添加物  超高效液相色谱串联质谱法

英文关键词:health-care food  illegal additives  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立一种超高效液相色谱串联质谱法同时测定保健食品中66种非法添加物。方法 样品用甲醇进行超声提取, 利用超高效液相色谱质谱联用技术于完成检测, 并利用软件对图谱进行分析, 并对市售液体类、半固体类、粉末类及片剂4类保健品进行测定。结果 各组分在 1～200 ng/mL浓度范围内具有良好的线性(r2均大于0.993), 各化合物的检出限为7.8~42.5 μg/kg。该方法加标量为50、100、250 μg/kg时, 精密度为0.5%~26.3%、目标物的回收率为66.3%~120.4%(n=8)。结论 该方法准确可靠、简便快速, 可适用于筛查保健品中非法添加药物的抽检工作, 能够减少工作量, 提高检测效率, 节省资源。

英文摘要:

      Objective To establish a method for simultaneous determination of 66 illegal additives in health-care food by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods The samples were extracted with methanol by ultrasound, detected by UPLC-MS/MS, and analyzed by software. Four kinds of health products including liquid, semi-solid, powder and tablet on market were determined. Results Each component has good linearity in the concentration range of 1–200 ng/mL (r2>0.993). The detection limit of each compound was 7.8–42.5 μg/kg and the precision of the method was 0.5%-26.3% and the recovery rate of the target was 66.3%–120.4% (n=8) when the scalar was 50, 100, 250 μg/kg. Conclusion The method is accurate, reliable, simple and fast, and can be applied to the screening of drugs illegally added in health products, which can reduce the workload, improve the detection efficiency and save resources.