卡尔费休容量法测定蜂花粉水分含量

Research on the determination of water content in bee pollen samples by volumetric Karl Fischer method

中文关键词:  蜂花粉  卡尔费休容量法  水分含量

英文关键词:bee pollen  volumetric Karl Fisher method  water content

中文摘要:

      目的 建立卡尔费休容量法测定蜂花粉水分含量。方法 蜂花粉经机械粉碎并研磨后过30目筛, 选用甲醇-甲酰胺-正辛醇(1:1:1, V/V/V)作为反应溶剂, 采用卡尔费休容量法测定蜂花粉的水分含量, 并同时与65 ℃减压干燥法及105 ℃直接干燥法进行比较。结果 卡尔费休容量法测得的蜂花粉水分含量更能代表蜂花粉中的真实水分, 加标回收率在84.36%～98.45%之间, 相对标准偏差小于2%(n=7)。结论 该方法操作简单方便、重现性好, 可以用于蜂花粉中水分含量的测定工作。

英文摘要:

      Objective To establish a method for the determination of water content in bee pollen by volumetric Karl Fischer (KF) method. Methods Bee pollen was mechanically crushed and ground to 30 mesh sieve. The water content of bee pollen was determined by volumetric KF method with methanol-formamide-n-octanol (1:1:1, V/V/V) as the reaction solvent, and compared with vacuum drying method at 65 ℃ and direct drying method at 105 ℃. Results The water content of bee pollen determined by volumetric KF method was more representative of the real water content in bee pollen. The recovery rate ranged from 84.36% to 98.45%, and the relative standard deviation was less than 2% (n=7). Conclusion The volumetric KF method is easy to operate and has good reproducibility, which can be used for the determination of water content in bee pollen.