液相色谱-高分辨质谱法测定豆芽中4-氯苯氧乙酸的不确定度评估

Uncertainty evaluation for determination of 4-chlorophenoxyacetic acid in bean sprouts by liquid chromatography- high resolution mass spectrometry

中文关键词:  液相色谱串联高分辨质谱法  豆芽  4-氯苯氧乙酸  不确定度

英文关键词:liquid chromatography- high resolution mass spectrometry  bean sprouts  4-chlorophenoxyacetic acid  uncertainty

中文摘要:

      目的 评定液相色谱串联高分辨质谱法测定豆芽中4-氯苯氧乙酸含量的不确定度。方法 按照SN/T 3725-2013《出口食品中4-氯苯氧乙酸残留量的测定》测定豆芽中4-氯苯氧乙酸, 根据JJF 1059.1-2012《测定不确定度评定与表示》的方法, 建立高分辨液质联用法测定豆芽中4-氯苯氧乙酸不确定度评定的数学模型, 分析其中各不确定度分量的来源, 并对其分别进行量化, 最终合成豆芽中4-氯苯氧乙酸含量测定结果的不确定度。结果 豆芽中4-氯苯氧乙酸含量测定结果为35.11 μg/kg时, 在95%的置信区间下, 扩展不确定度为4.28 μg/kg (k=2)。结论 标准溶液的配制是实验过程不确定度主要来源。

英文摘要:

      Objective To evaluate the uncertainty for the determination of 4-chlorophenoxyacetic acid in bean sprouts by liquid chromatography- high resolution mass spectrometry. Methods According to SN/T 3725-2013 Determination of 4-chlorophenoxyacetic acid residues in food for export, content of 4-chlorophenoxyacetic acid in bean sprouts was detected. According to JJF 1059.1-2012 Evaluation and expression of measurement uncertainty, a mathematical model for the evaluation of the uncertainty of 4-chlorophenoxyacetic acid in bean sprouts by high-resolution liquid chromatography-mass spectrometry was established, the sources of each uncertainty component was analyzed and quantified, and 4-chlorophenoxy in bean sprouts uncertainty in the determination of acetic acid content was synthesized. Results When the content of 4-chlorophenoxyacetic acid in bean sprout was 35.11 μg/kg, the expanded uncertainty was 4.28 μg/kg (k=2) under 95% confidence interval. Conclusion The preparation of standard solution is the main source of uncertainty in experimental process.