马兰头总黄酮的大孔树脂纯化工艺优化

Optimization of purification process of total flavonoids from Kalimeris by macroporous resin

中文关键词:  马兰头  总黄酮  大孔树脂  纯化  响应面法

英文关键词:Kalimeris  total flavonoids  macroporous resin  purification  response surface method

中文摘要:

      目的 利用大孔树脂来纯化马兰头中粗黄酮, 并确定纯化黄酮的最佳工艺。方法 以黄酮回收率为指标, 在单因素实验的基础上运用Box-Behnken响应面法(response surface methodology, RSM)设计三因素三水平实验以获得最佳纯化条件。结果 HPD-600大孔吸附树脂纯化马兰头粗提液的最佳工艺条件为: 上样浓度0.93 mg/mL、上样pH为3.00、洗脱剂体积分数为84.17%、吸附速率1 BV/h, 洗脱速率1 BV/h, 此条件下马兰头总黄酮的质量分数由纯化前的4.11%提高到纯化后的50.80%。结论 利用HPD-600型大孔树脂可以较好地纯化马兰头中的总黄酮。

英文摘要:

      Objective To purify crude flavonoids from Kalimeris by macroporous resin and determine the optimum purifying process for flavonoids. Methods Based on the single factor experiment, Box-Behnken response surface methodology, RSM) was used to design a three-factor and three-level experiment to obtain the best purification conditions with flavone recovery rate as the index. Results The optimum conditions for purification of crude extract of Kalimeris with HPD-600 macroporous adsorption resin were as follows: loading concentration 0.93 mg/mL, loading pH 3.00, eluent volume fraction 84.17%, adsorption rate 1 BV/h, and elution rate 1 BV/h. Under these conditions, the mass fraction of total flavonoids in Kalimeris was increased from 4.11% before purification to 50.80% after purification. Conclusion HPD-600 macroporous resin can be used to purify the total flavonoids in Kalimeris.