沙门氏菌在不同品牌显色培养基上的对比分析

Comparative analysis of Salmonella on different brands of chromogenic medium

中文关键词:  沙门氏菌  显色培养基  对比分析

英文关键词:Salmonella  chromogenic medium  comparative analysis

中文摘要:

      目的 对比分析不同品牌的沙门氏菌显色培养基, 评判其特异性、选择性和灵敏度。方法 采用不同种的沙门标准菌株及样品分离菌株, 分别划线不同品牌沙门氏菌显色培养基, 通过观察菌落形态, 以验证其特异性、选择性、灵敏度(假阴性和假阳性)情况。结果 在特异性与选择性验证试验中, A、B、C品牌沙门氏菌显色培养基不易区分恶臭假单胞菌、铜绿假单胞菌, 容易与鼠伤寒沙门氏菌混淆; 在假阴性试验中, A、B、C、D、E、F 6种不同品牌沙门氏菌显色培养基均无差异; 在假阳性试验中D、E、F品牌沙门氏菌显色培养基可以有效区别常见干扰菌泛生菌属、肺炎克雷伯肺炎亚种和奇异变形杆菌等的影响, 出现假阳性的概率相对较低; 亚硫酸铋琼脂平板培养基可以在实验中起到很好的排除干扰作用。结论 不同品牌的同种培养基的选择性、准确率差异较大, 质量优劣存在差异, 实用性也有所不同, 在实验时尽量选择不同品牌的培养基, 避免因培养基的质量差异而出现检验结果的错判。

英文摘要:

      Objective To compare and analyze the different brands of Salmonella chromogenic medium, and evaluate their specificity, selectivity and sensitivity. Methods Salmonella standard strains and samples isolated from different species were used to underline on the different brands of Salmonella chromogenic medium , and the colony morphology was observed to verify the specificity, selectivity and sensitivity (false-negative and false-positive). Results In the specificity and selectivity verification test, the Salmonella chromogenic medium of brands A, B, and C was not easy to distinguish between Pseudomonas putida and Pseudomonas aeruginosa, and was easily confused with Salmonella typhimurium. In the false-negative test, there was no difference between A, B, C, D, E, F six different brands of Salmonella chromogenic medium. In the false-positive test, the chromogenic medium of Brand D, E and F could effectively distinguish the influence of common interfering bacteria such as Pantoea, Klebsiella pneumoniae subspecies and Proteus mirabilis, and the probability of false positive was relatively low. Bismuth sulfite agar plate medium could play a good role in eliminating interference in the experiment. Conclusion Different brands of the same culture medium have different selectivity, accuracy, quality and practicability. In the experiment, it should be try to choose different brands of culture medium to avoid the wrong judgment of the test results due to the different quality of culture medium.