超高效液相色谱-串联质谱法同时测定鸡蛋中7种色素

Simultaneous determination of 7 kinds of pigments in egg by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  色素  超高效液相色谱-串联质谱  鸡蛋

英文关键词:pigment  ultra performance liquid chromatography-tandem mass spectrometry  egg

中文摘要:

      目的 建立同时测定鸡蛋中7种色素含量的超高效液相色谱串联质谱(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)法。方法 鸡蛋样品用乙腈超声提取。经Phenomenex Kinetex F5(100 mm×3.0 mm, 2.6 μm)色谱柱分离, 流动相A: 0.1%的甲酸/水溶液; 流动相B: 0.1%的甲酸/乙腈溶液作为流动相梯度洗脱。电喷雾离子源(electrospray ionization, ESI)正离子多反应监测模式定量分析。结果 7种色素在浓度1～20 μg/L范围内线性关系良好, 相关系数r2≥0.998。叶黄素、角黄素、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ的检出限均为0.1 μg/kg, 核黄素的检出限为0.2 μg/kg。7种色素在加标量2～10 μg/kg范围内, 回收率为80.2%~106.7%, 相对标准偏差为1.5%~6.0%。结论 该方法准确、快速、高效, 可用于鸡蛋中色素的定性、定量分析。

英文摘要:

      Objective To establish the method for the simultaneous determination of 7 pigments in eggs by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Egg samples were extracted by acetonitrile ultrasound, and separated by Phenomenex Kinetex F5 (100 mm×3.0 mm, 2.6 m) column. Mobile phase A: 0.1% formic acid/aqueous solution and mobile phase B: 0.1% formic acid/acetonitrile solution were used as mobile phase for gradient elution. Positive ion multi-reaction monitoring mode of electrospray ionization (ESI) was used for quantitative analysis. Results The linearity of the 7 pigments was good in the range of 1-20 g/L, and the correlation coefficient r2≥0. 998. The detection limits of lutein, canthaxanthin, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ and Sudan Ⅳ were 0.1 g/kg, and that of riboflavin was 0.2 g/kg. The recoveries of 7 pigments were 80.2%-106.7% and the relative standard deviations were 1.5%-6.0% in the range of 2-10 g/kg. Conclusion The proposed method is fast, accurate and efficiency, which is suitable for qualitative and quantitative analysis of endogenous and exogenous pigments in eggs.