博落回生物碱提取工艺优化

Optimization of extraction of alkaloids from Macleaya cordata

中文关键词:  博落回  生物碱  高效液相色谱法

英文关键词:Macleaya cordata  alkaloid  high performance liquid chromatography

中文摘要:

      目的 探讨优化博落回叶片中生物碱的提取方法。方法 采用甲醇提取法、超声-甲醇提取法、超声-甲醇补足提取法、超声-乙醇提取法和乙醇提取法5种方法提取博落回叶片中的生物碱, 采用高效液相色谱法(high performance liquid chromatography, HPLC)测定其中原阿片碱(protopine, PRO)、别隐品碱(allocryptopine, ALL)、血根碱(sanguinarine, SAN)和白屈菜红碱(chelerythrine, CHE)4种生物碱的含量。结果 超声-甲醇补足提取法提取光泽县野生博落回叶中的总生物碱含量最高, 为(104.939±0.449) mg/g。三明明溪、南平光泽2地博落回叶片均有4种生物碱, 所有提取方法提取的生物碱含量都满足ALL＞PRO＞SAN≈CHE。超声与回流相比提取率更高, 乙醇为提取溶剂时, 超声辅助的影响较小。结论 超声-甲醇补足提取法为博落回生物碱的最优提取方法, 此方法适用于博落回中总生物碱的提取。

英文摘要:

      Objective To optimize a extraction method of alkaloids from Macleaya cordata. Methods The extraction methods of alkaloids are methanol extraction, ultrasonic assisted methanol extraction, ultrasonic assisted methanol complement extraction, ultrasonic assisted ethanol extraction, and ethanol extraction. The contents of protopine, allocryptopine, sanguinarine, and chelerythrine were analyzed by high performance liquid chromatography (HPLC). Results The highest total alkaloid content was (104.939±0.449) mg/g in the leaves of wild Macleaya cordata from Guanze by ultrasonic assisted methanol complement extraction. Four alkaloids were detected in leaves of Macleaya cordata from Mingxi county of Sanming and Guangze county of Nanping, the alkaloid content have the same trendy: ALL＞PRO＞SAN≈CHE. The extraction rate of ultrasonic extraction method was higher than reflux extraction method. When ethanol was used as the extraction solvent, the effect of ultrasound assisted was small. Conclusion The ultrasonic assisted methanol complement extraction method is the optimal extraction method of alkaloids from Macleaya cordata, and this method is applicable to the extraction of the total alkaloids from Macleaya cordata.