特殊医学用途配方食品和婴幼儿配方乳粉中氟含量测定方法的改进

Improvement of the determination method of fluorine in formula food for special medical use and infant formula milk powder

中文关键词:  氟含量  氟离子选择电极法  特殊医学用途配方食品  婴幼儿配方乳粉

英文关键词:fluorine content  fluoride ion selective electrode method  formula food for special medical purpose  infant formula milk powder

中文摘要:

      目的 对国标方法测定特殊医学用途配方食品和婴幼儿配方乳粉类产品中氟含量测定进行改进。 方法 样品经Taka淀粉酶酶解, 高氯酸溶液沉淀蛋白质, 加入总离子强度缓冲剂消除金属干扰离子, 过滤后, 使用氟离子选择电极测定样品中氟含量。结果 与国标法相比, 改进方法使测试溶液澄清, 电极响应更快, 回收率范围为92.8%~107.5%, 相对标准偏差为3.36%~4.38%, 能满足方法准确度和精密度要求。结论 改进后的方法能够满足方法学要求, 能够准确地检测特殊医学用途配方食品和婴幼儿配方乳粉类产品中的氟含量。

英文摘要:

      Objective To improve the national standard method for the determination of fluorine in formula food for special medical use and infant formula milk powder products. Methods The samples were enzymolysised by Taka amylase, the proteins were precipitated by perchloric acidsolution, the metal ion interference was masked by total ion strength buffer, and the fluorine in samples which were filtrated was detected by fluoride ion selective electrode. Results A clear solution was obtained by using the improved method, whose electrode response was more faster than the national standard method. The recovery was 92.8%?107.5%, and the relative standard deviation was 3.36%?4.38%, which could meet the accuracy and precision requirements of the method. Conclusion It is verified that the improved method could meet the requirements of methodology, and it can accurately detect the fluorine content in formula food for special medical purpose and infant formula milk powder products.