转基因食用农产品的快速检测方法

Rapid detection methods for genetically modified edible agricultural products

中文关键词:  转基因  农产品  基因  蛋白质  检测

英文关键词:transgenic  agricultural products  gene  protein  detection

中文摘要:

      商业化转基因农作物广泛种植, 转基因农产品被大量用作食品和动物饲料。我国和全球大部分国家都实行转基因生物强制标识制度。转基因农产品的现场监督检测急需稳定可靠的快速检测手段。本文论述了主要的基因水平检测方法包括PCR、基因芯片、环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)和蛋白质水平检测方法(蛋白质免疫印迹法、酶联免疫吸附法、胶体金免疫层析试纸条法)。通过比较分析上述各检测方法的优缺点, LAMP和胶体金试纸条法是当前最理想的现场快速检测方法。

英文摘要:

      Commercial genetically modified crops are widely planted, and genetically modified agricultural products are widely used in food and animal feed. China and most countries in the world have implemented the genetically modified organisms mandatory labeling system. On-site supervision urgently need stable and reliable rapid inspection methods on testing of genetically modified agricultural products. In this paper, the main methods of gene level detection were discussed, including PCR, gene chip, loop-mediated isothermal amplification (LAMP) and protein level detection methods (western blot, enzyme-linked immunosorbent assay, colloidal gold immunochromatography test strip). By comparing and analyzing the advantages and disadvantages of the above detection methods, LAMP and colloidal gold test strip method were currently the most ideal on-site rapid detection methods.