高效液相色谱-二极管阵列检测食用绿色素中叶绿素铜钠含量

Determination of sodium copper chlorophyllin in edible green pigments by high performance liquid chromatography-diode array

中文关键词:  高效液相色谱-二极管阵列  食用绿色素  叶绿素铜钠

英文关键词:high performance liquid chromatography-diode array  edible green pigment, sodium copper chlorophyllin

中文摘要:

      目的 建立高效液相色谱-二极管阵列检测食用绿色素中叶绿素铜钠含量的方法。方法 样品经超声处理后, 以0.5%(V/V)乙酸溶液/甲醇/丙酮为流动相, 经Agilent 5 TC-C18色谱柱进行梯度淋洗, 并于405 nm处进行定量检测。结果 在1~200 μg/mL线性范围内, 线性方程为Y=5.7834X–2.3787, 相关系数r2=0.99938, 方法检出限为0.21 mg/kg, 相对标准偏差为1.4%。对7份食用绿色素样品进行检测, 均未发现叶绿素铜钠成分。结论 此方法实现了对食用绿色素中叶绿素铜钠含量的定性和定量分析, 与传统紫外分光光度检测法相比, 灵敏度和准确性有效提高。

英文摘要:

      Objective To establish a method for determination of sodium copper chlorophyllin in edible green pigments by high performance liquid chromatography-diode array. Methods After ultrasonic treatment, the samples were eluted by acetic acid solution(0.5%, V/V)/methanol/acetone, and then separated by Agilent TC-C18 silica gel column. Quantitative detection was carried out at wavelength of 405 nm. Results The linear correlation was good in the concentration range of 1–200 μg/mL(r2=0.9998). The limit of detection was 0.21 mg/kg and the relative standard deviation was 1.4%. No sodium copper chlorophyllin was found in 7 edible green pigments samples. Conclusion This method realized the qualitative and quantitative analysis of sodium copper chlorophyllin in edible green pigments. Compared with the traditional ultraviolet spectrophotometry, the sensitivity and accuracy is improved effectively.