高效液相色谱法同时测定红心鸭蛋中5种类胡萝卜素含量

Simultaneous determination of 5 kinds of carotenoids in red yolk of duck’s eggs by high performance liquid chromatography

中文关键词:  高效液相色谱法  红心鸭蛋  类胡萝卜素

英文关键词:high performance liquid chromatography  red yolk of duck's eggs  carotenoids

中文摘要:

      目的 建立高效液相色谱法同时测定红心鸭蛋中辣椒红素、虾青素、叶黄素、玉米黄素、角黄素5种类胡萝卜素的方法。方法 样品经乙腈提取, 浓缩后用正己烷脱脂。采用Agilent Poroshell 120 EC-C18 (4.6 mm×100 mm, 4 μm)色谱柱分离, 以20 mmol/L乙酸铵和乙腈作为流动相进行梯度洗脱, 流速1.0 mL/min, 二极管阵列检测器, 波长471 nm, 25 min完成检测。结果 5种类胡萝卜素可以实现基线分离, 线性关系良好, 相关系数在均在0.999以上, 样品在3个浓度水平的加标回收率为84.39%~93.42%(n=6)。结论 该方法简便、快捷、准确, 可用于红心鸭蛋中5种类胡萝卜素的测定。

英文摘要:

      Objective To establish a method for simultaneous determination of 5 carotenoids including capsaicin, astaxanthin, lutein, zeaxanthin and capsaicin in duck eggs with red hearts by high performance liquid chromatography. Methods Samples were extracted with acetonitrile. After being concentrated, the acetonitrile phase was defatted by hexane. Chromatographic separation was performed on an Agilent Poroshell 120 EC-C18(4.6 mm×100 mm, 4 μm)column with acetonitrile-20 mmol/L ammonium acetate as mobile phase by gradient elution. The flow-rate was 1.0 mL/min. Photo-diode array detector was applied and was set at 471 nm. The analysis process could be completed in 25 min. Results The results showed that the baseline separation was obtained for the 5 carotenoids. All the 5 carotenoids had excellent linear relationships (r>0.999). The recoveries of samples at 3 concentration levels were 84.39%?93.42% (n=6). Conclusion This method is simple, rapid, highly sensitive and with good repeatability. It can be used for determination of 5 carotenoids in red yolk of duck's eggs.