固相萃取-高效液相色谱-紫外检测法测定蔬菜中阿维菌素的残留量

Determination of avermectin residue in vegetables by solid-phase extraction with ultraviolet detection coupled to high performance liquid chromatography

中文关键词:  蔬菜  阿维菌素  固相萃取  高效液相色谱-紫外检测法

英文关键词:vegetables  avermectin  solid-phase extraction  high performance liquid chromatography with ultraviolet detection

中文摘要:

      目的 建立固相萃取—高效液相色谱法-紫外检测法测定蔬菜中阿维菌素残留量的方法。方法 选取本地区市售的6种蔬菜样品, 经乙酸乙酯提取, 经固相萃取柱净化后用甲醇定容。在C18色谱柱上进行分离, 以甲醇-水(9: 1,体积比)为流动相, 流速1.0 mL/min, 检测波长为245 nm。结果 在0.05～1.00 μg/mL浓度范围内, 方法线性关系良好, r2值为0.9999, 定量限为0.01 mg/kg。平均回收率为94.0%～98.0%, 相对标准偏差不超过6%(n=6)。结论 本方法前处理过程简便高效, 样品检出限低, 灵敏度较高, 适用于蔬菜中阿维菌素残留量的检测。

英文摘要:

      Objective To establish a method for the determination of avermectin residue in vegetables using solid-phase extraction (SPE) and high performance liquid chromatography with ultraviolet detection (HPLC-UV). Methods Six kinds of samples collected from supermarket were extracted with ethylacetate, then the further cleanup was performed on a solid phase extraction cartridge and then fixed volume by methanol. The analysis was carried out by C18 reverse phase column, with the flow rate of the mobile phase (methanol-water, V/V=9:1) 1.0 mL/min, the detection wavelength 245nm and the column temperature 40 ℃. Results In the concentration range of 0.05-1.00 μg/mL, the liner relationship of the method was good(r2=0.9999) and the quantification limit was 0.01 mg/kg. The average recovery rate was 94.0%-98.0%, and the relative standard deviation (RSD) was less than 6%(n=6). Conclusion This method has simple pretreatment, good stability and reliability, good qualitative and quantitative analysis ability, which is suitable for detecting avermectin residue in vegetables.