液相色谱法测定油脂及其制品中苯并(a)芘含量

Determination of benzo(a)pyrene in fats and oils and their products by liquid chromatography

中文关键词:  液相色谱法  油脂及其制品  苯并(a)芘

英文关键词:liquid chromatography  fats and oils and their products  benzo(a)pyrene  fats  oils

中文摘要:

      目的 建立液相色谱法测定油脂及其制品中苯并(a)芘的方法。方法 试样经过正己烷提取, 中性氧化铝或分子印迹小柱净化, C18色谱柱分离, 荧光检测器检测。结果 方法的线性范围为0.5~20 μg/L, 线性相关系数为0.9999, 最低定量浓度(the limit of quantity, LOQ)为0.5 μg/kg, 在0.5、5、15 μg/kg 3个添加水平下平均回收率为75.5%~93.8%, 相对标准偏差(relative standard deviation, RSD)为3.0%~9.6%。结论 该方法准确度与精密度满足GB/T 27404规定, 定量限满足GB 2762实施要求, 被用于修订GB/T 5009.27-2003为 GB 5009.27-2016。

英文摘要:

      Objective To establish a method for determination of benzo(a)pyrene in fats and oils and their products samples by liquid chromatography (LC). Methods The samples were extracted by N-hexane, purified by neutral alumina or molecularly imprinted column, separated by C18 column and detected by fluorescence detector. Results The method showed a good linearity in the range of 0.5?20 μg/L with correlation coefficients of 0.9999, and the limit of quantitation (LOQ) was 0.5 μg/kg. The average recoveries were 75.5%?93.8% at the spiked level of 0.5, 5, 15 μg/kg with the relative standard deviation (RSD) of 3.0%?9.6%. Conclusion The accuracy and precision of this method meet the requirements of GB/T 27404. The limit of quantitation meets the implementation requirements of GB 2762. This method has been used to revise GB/T 5009.27-2003 to GB 5009.27-2016.