高效液相色谱法同时测定芹菜籽提取物中芹菜苷和3-甲氧基芹菜苷的含量

Simultaneously determination of apigenin and 3- methoxyapigenin content in celery seed extract by high performance liquid chromatography

中文关键词:  芹菜籽  芹菜苷  3-甲氧基芹菜苷  高效液相色谱

英文关键词:celery seed  apigenin  3-methoxyapigenin  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定芹菜籽提取物中芹菜苷和3-甲氧基芹菜苷含量的分析方法。方法 以1%乙酸溶液:乙腈=85:15(V:V)为流动相, 梯度洗脱, 样品经C18色谱柱(250 mm×4.6 mm, 5 μm)分离, 并于330 nm波长检测。结果 芹菜苷在0.00334~0.03340 mg/mL之间呈现良好的线性关系, r2=0.9998, 平均回收率为95.2%~104.5%, 相对标准偏差为2.0%; 3-甲氧基芹菜苷在0.00367~0.0367 mg/mL之间呈现良好的线性关系, r2=0.9999, 平均回收率在96.6%~104.0%之间, 相对标准偏差为1.9%, 均符合标准GB/T 27404-2008实验室质量控制规范食品理化检测中的要求。结论 该方法操作简便, 准确, 重现性好, 能同时测定芹菜苷和3-甲氧基芹菜苷的含量, 可在实验室推广使用。

英文摘要:

      Objective To establish a method for simultaneous determination of apigenin and 3-methoxyapigenin by high performance liquid chromatography (HPLC). Methods With 1% acetic acid solution: acetonitrile=85:15 (V:V) as the mobile phase and performing gradient elution, the samples were separated by C18 column (250 mm× 4.6 mm, 5μm) and detected at the wavelength of 330 nm. Results Apigenin had a good linear relationship in range of 0.00334?0.03340 mg/mL (r2=0.9998). The average recoveries were 95.2%?104.5%, with the RSD of 2.0%. 3-methoxyapariin had a good linear relationship in range of 0.00367?0.0367 mg/mL (r2=0.9999), and average recoveries were 96.6%?104.0%, with the RSD of 1.9%. All the results met the requirements of the standard GB/T 27404-2008. Conclusion This method is simple, accurate, and has a good reproducibility, which can be used for the simultaneous determination of apigenin and 3-methoxy apigenin, and can be popularized in the laboratories.