火焰原子吸收光谱法和电感耦合等离子体发射光谱法测定黄酒中氧化钙含量的方法对比研究

Comparative of determination of calcium oxide in yellow rice wine by flame atomic absorption spectrometry and inductively coupled plasma optical emission spectrometry

中文关键词:  火焰原子吸收光谱法  电感耦合等离子体发射光谱法  黄酒  氧化钙

英文关键词:flame atomic absorption spectrometry  inductively coupled plasma emission spectrometry  yellow rice wine  calcium oxide

中文摘要:

      目的 比较火焰原子吸收光谱法(flame atomic absorption spectrometry, FAAS)和电感耦合等离子体发射光谱法(inductively coupled plasma optical emission spectrometry, ICP-OES)测定黄酒中的氧化钙含量。 方法 将黄酒样品、加标试样样品、质控样品通过微波消解仪消解, 定容后分别用火焰原子吸收分光光度计, 电感耦合等离子体发射光谱仪测定样品中钙的含量, 并对2种方法的线性范围、方法检出限、精密度和回收率进行考察。结果 FAAS法的方法检出限和精密度分别为0.10 μg/mL和0.0223 g/L, ICP-OES法的方法检出限和精密度分别为0.015 μg/m和0.00873 g/L, ICP-OES法有更低的检出限和更高的精密度, 2者检测样品结果相对标准偏差(RSD)在3%以内, 回收率在96.4%~102.6%之间, 相关系数r为0.9995~0.9999, 这2种方法中标准参考物质菠菜(GSB-6)中的钙含量的测定值在标准参考范围内。结论 FAAS法测定黄酒中的氧化钙, 前处理操作复杂, 并且酸度对测定结果有较大影响, 同时该法的检出限较高和精密度较低, 而ICP-OES法线性关系好, 检出限低, 稳定性好, 回收率高, 测定结果准确, 因此该法更适合黄酒中氧化钙的测定。

英文摘要:

      Objective To compare the determination of calcium oxide in yellow rice wine by flame atomic absorption spectrometry (FAAS) and inductively coupled plasma optical emission spectrometry (ICP-OES). Methods The samples of yellow rice wine, labeled samples and the quality control sample were digested by microwave digester. The content of calcium in the sample was determined by flame atomic absorption spectrophotometer and inductively coupled plasma emission spectrometer after constant volume. And the linear range, the method detection limit, precision and recovery of the 2 methods were investigated. Results The limit of detection and precision of FAAS were 0.10μg/mL, 0.0223 g/L, respectively. The limit of detection and precision of ICP-OES were 0.015 μg/mL, 0.00873 g/L, respectively. ICP-OES had lower detection limit and higher precision. The relative standard deviation (RSD) of the 2 samples was within 3%. The recovery rate ranged from 96.4% to 102.6%, and the correlation coefficient r was 0.9995?0.9999. The calcium content of spinach (GSB-6) was within the standard reference range by this 2 methods. Conclusion FAAS method for the determination of calcium oxide in yellow rice wine has a complicated pretreatment operation, and the acidity has a great influence on the determination results. Meanwhile, the detection limit and precision of the method are relatively high. While ICP-OES has strong linear relation, low detection limit, high stability, high recovery and accurate measurement results, so this method is more suitable for the determination of calcium oxide in yellow rice wine.