高效液相色谱法测定板栗种子中的γ-氨基丁酸

Determination of γ-amino butyric acid in chestnut seed by high performance liquid chromatography

中文关键词:  高效液相色谱法  γ-氨基丁酸  板栗种子

英文关键词:high performance liquid chromatography  γ-amino butyric acid  chestnut seeds

中文摘要:

      目的 建立高效液相色谱法测定板栗种子中γ-氨基丁酸的分析方法。方法 板栗样品使用2,4-二硝基氟苯乙腈进行衍生化前处理后, 以磷酸缓冲溶液(pH=7)、乙腈和水为流动相, 采取梯度洗脱的方式进行测定, 柱温35 ℃, 流速1.0 mL/min, 进样量20 μL, 使用紫外检测器, 测定波长为360 nm。结果 本方法标准曲线呈现良好的线性关系, 相关系数达到0.9996。方法的回收率为109%, 相对标准偏差为0.4%, γ-氨基丁酸最小检出限0.05 μg/mL。精密度实验得出5次进样的相对标准偏差为0.55%。对3种不同品种的板栗种子进行测定时, 3种样品的RSD分别为3.077%、3.219%、5.333%, 平行性较好。结论 该方法快速、易操作, 具有较高的测量精密度和准确度, 对实际样品进行测试时能避免杂质峰的干扰, 重现性好, 能够满足对板栗种子中γ-氨基丁酸含量的检测要求。

英文摘要:

      Objective To establish a method for the determination of teraminobutyric acid in chestnut seeds by high performance liquid chromatography. Methods After the sample was derivatized by 2, 4- dinitrofluorobenzene, it was determined by gradient elution with phosphate buffer solution (pH=7), acetonitrile and water as mobile phases. The column temperature was selected at 35 ℃, the flow rate was 1.0 mL/min, the injection volume was 20 μL, and the detection wavelength was 360 nm with ultraviolet detector. Results The standard curve of this method showed a good linear relationship with a correlation coefficient of 0.9996. The recoveries were 109%, the relative standard deviations was 0.4%, and the limit of quantitation was 0.05 μg/mL. The precision experiment showed that the relative standard deviation of 5 injections was 0.55%. When the seeds of 3 different varieties of chestnut were measured, the RSD of the three samples were 3.077%, 3.219%, and 5.333%, respectively, with good parallelism. Conclusion The proposed method is fast and easy to operate, with high precision and accuracy of measurement, which can avoid the interference of impurity peaks when testing actual samples. It has good reproducibility, and is suitable for detecting γ-amino butyric acid in chestnut seeds.