微波水解-茚三酮柱后衍生离子交换色谱法测定鹌鹑蛋中的17种氨基酸

Determination of 17 kinds of amino acids in quail eggs by microwave hydrolysis-ninhydrin column post-derivative ion exchange chromatography

中文关键词:  微波水解  氨基酸  茚三酮柱后衍生离子交换色谱法

英文关键词:microwave hydrolysis  amino acid  ninhydrin column post-derivative ion exchange chromatography

中文摘要:

      目的 建立微波水解茚三酮柱后衍生离子交换色谱法同时测定鹌鹑蛋中17种氨基酸含量的分析方法。方法 采用磺酸型阳离子树脂离子交换柱分离, 茚三酮溶液作为柱后衍生剂, 紫外可见分光光度检测器进行检测, 检测波长为570 nm和440 nm, 并对微波水解时间及温度进行条件优化。结果 17种氨基酸在一定范围内呈良好线性关系, 相关系数均大于0.99; 检出限为0.376~0.906 mg/L。结果表明, 150 ℃下微波水解 20 min的结果与传统加热水解(110 ℃, 22 h)的效果基本相同。结论 该方法分析时间较短, 灵敏度较高, 可用于鹌鹑蛋中氨基酸含量的检测。

英文摘要:

      Objective To establish a method for simultaneous determination of 17 amino acids in quail eggs by microwave-hydrolyzed ninhydrin column post-derivative ion exchange chromatography. Methods The sulfonium-type cationic resin ion exchange column was used for separation, and the ninhydrin solution was used as a post-column derivatizer. The UV-visible spectrophotometer was used for detection. The detection wavelengths were 570 nm and 440 nm, and the microwave hydrolysis time and temperature were optimized. Results The 17 amino acids showed a good linear relationship in the range of 0.005?1.000 μmol/mL, and the correlation coefficient was greater than 0.99. The limits of detection were 0.376?0.906 mg/L. The results of microwave hydrolysis at 150 ℃ for 20 min were basically the same as those of traditional heating hydrolysis (110 ℃, 22 h). Conclusion This method has short analysis time and high sensitivity, and can be used for detecting the amino acid content in quail eggs.