瘤果黑种草子总黄酮成分对结肠癌细胞株SW480增殖、迁移能力的影响及机制

Effects of total flavonoids from tumor fruit black grass on the proliferation and migration of colon cancer cell line SW480 and its mechanism

中文关键词:  瘤果黑种草子  总黄酮  人结肠癌细胞株SW480  细胞增值  细胞迁移  自噬相关分子

英文关键词:Nigellaglandulifera Preynet Sint  total flavonoids  human colon cancer cell line SW480  cell proliferation  cell migration  autophagy associated molecules

中文摘要:

      目的 探讨瘤果黑种草子总黄酮成分对人结肠癌细胞株SW480增殖、迁移能力的影响及可能的作用机制。方法 用不同浓度的瘤果黑种草子总黄酮成分药物干预的结肠癌细胞株SW480, MTT法检测SW480细胞株的增殖能力, 倒置显微镜观察对结肠癌细胞株SW480细胞形态的影响, 划痕实验检测对细胞的迁移能力的影响, 实时荧光定量PCR检测瘤果黑种草子总黄酮成分对细胞中BECN1、MAPILC3B基因表达的影响。结果 瘤果黑种草子总黄酮对人结肠癌细胞株SW480体外增殖的抑制作用明显, 并呈浓度依赖性抑制人结肠癌细胞株SW480的增殖, 在25 μg/mL时达到最大的抑制率, 抑制率为81.28%。倒置显微镜观察, 用瘤果黑种草子总黄酮成分药物干预后, 人结肠癌细胞株SW480形态学发生明显变化, 瘤果黑种草子总黄酮成分可抑制人结肠癌细胞株SW480迁移能力, 同时, 可上调自噬相关基因BECN1、MAPILC3B的表达量。结论 瘤果黑种草子总黄酮成分可抑制人结肠癌细胞株SW480的增殖、迁移能力, 机制可能与能影响细胞中BECN1、MAPILC3B基因表达量有关。

英文摘要:

      Objective To investigate the effects of total flavonoids from Nigellaglandulifera preynet Sint on proliferation and migration of human colon cancer cell line SW480 and its possible mechanism. Methods The colon cancer cell line SW480 was treated with different concentrations of total flavonoids from Nigellaglandulifera preynet Sint, its proliferation ability was detected by MTT method, its morphological changes were observed by inverted microscope, its migration ability was detected by scratch test, and the effects of total flavonoids on BECN1 and MAPILC3B gene expression in cells were detected by real-time fluorescence quantitative PCR. Results The total flavonoids of Nigellaglandulifera preynet Sint significantly inhibited the proliferation of human colon cancer cell line SW480 in vitro, and inhibited the proliferation of human colon cancer cell line SW480 in a concentration-dependent manner. The inhibition rate reached the maximum at 25 μg/mL, with an inhibition rate of 81.28%. The morphological changes of human colon cancer cell line SW480 were observed by inverted microscopy and they were obvious. The total flavonoids of Nigellaglandulifera preynet Sint could inhibit the migration of human colon cancer cell line SW480 and increase the expression of autophagy related genes BECN1 and MAPILC3B. Conclusion Total flavonoids from Nigellaglandulifera preynet Sint can inhibit the proliferation and migration of human colon cancer cell line SW480. The mechanism may be related to the influence of BECN1 and MAPILC3B gene expression in cells.