新疆蔷薇红景天总黄酮抗炎作用及急性毒性研究

Anti-inflammatory activity and acute toxicity of the total flavonoids from Rhodiola rosea

中文关键词:  新疆蔷薇红景天  抗炎活性  急性毒性试验

英文关键词:Rhodiola rosea  anti-inflammatory activity  acute toxicity test

中文摘要:

      目的 研究新疆蔷薇红景天总黄酮的抗炎作用及其急性毒性。方法 昆明种小鼠60只, 随机分为模型组、阿司匹林组(0.2 g/kg)、总黄酮低、中、高剂量组(1.0、2.0、4.0 g/kg), 建立二甲苯致小鼠耳肿胀急性炎症模型, 考察蔷薇红景天总黄酮对二甲苯致小鼠耳肿胀的影响; SD大鼠50只, 随机分为模型组、阿司匹林组(0.14 g/kg), 总黄酮低、中、高剂量组(0.7、1.4、2.8 g/kg), 建立棉球诱发大鼠肉芽肿慢性炎症模型, 考察蔷薇红景天总黄酮对大鼠棉球肉芽肿形成的抑制作用; 酶联免疫检测试剂盒测定慢性炎症大鼠血清中肿瘤坏死因子(tumor necrosis factor-α, TNF-α)、环氧化酶-2(cyclooxygenase-2, COX-2)、白介素(interleukin-6, IL-6)、丝裂原激活蛋白激酶(mitogen-activated protein kinase, MAPK)4种炎症因子的含量, 考察蔷薇红景天总黄酮的抗炎作用; 选取20只昆明种小鼠, 随机分为实验组(160 g/kg)和空白组(蒸馏水), 灌胃连续给药2 w, 观察蔷薇红景天总黄酮的急性毒性。结果 与模型组比较, 蔷薇红景天总黄酮低、中、高剂量组对二甲苯致小鼠耳肿胀均有显著抑制作用(P<0.05, P<0.01, P<0.01), 其抑制率分别为25.75%、38.87%和61.13%; 蔷薇红景天总黄酮低、中、高剂量组对大鼠棉球肉芽肿的形成均有明显抑制作用(P<0.05, P<0.01, P<0.01), 其抑制率分别为42.06%、53.89%、54.15%; 蔷薇红景天总黄酮低、中、高各剂量组均能显著降低大鼠血清COX-2(P<0.01)和MAPK的含量(P<0.05, P<0.01, P<0.01); 其中、高剂量组能显著降低大鼠血清TNF-α和IL-6的含量(P<0.05, P<0.01)。结论 蔷薇红景天总黄酮具有一定抗急、慢性炎症作用, 并且安全无毒。

英文摘要:

      Objective To investigate the anti-inflammatory activity and acute toxicity of the total flavonoids from Rhodiola rosea in Xinjiang. Methods Totally 60 Kunming mice were randomly divided into model group, aspirin group (0.2 g/kg), low, medium and high doses of total flavonoids (1.0, 2.0, 4.0 g/kg). The acute inflammation model of ear swelling induced by xylene was established to investigate the effect of total flavonoids of Rhodiola rosea on ear swelling induced by xylene in mice. Totally 50 SD rats were randomly divided into model group, aspirin group (0.14 g/kg), low, medium and high doses of total flavonoids (0.7, 1.4, 2.8 g/kg). The chronic inflammation model of granuloma induced by cotton ball in rats was established to investigate the inhibitory effect of total flavonoids from Rhodiola rosea on granuloma formation in cotton pellets of rats. The levels of inflammatory factors of tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6) and mitogen-activated protein kinase (MAPK) in serum of chronic inflammatory rats were determined by enzyme-linked immunosorbent assay kit, the anti-inflammatory effects of total flavonoids from Rhodiola rosea were investigated. A total of twenty Kunming mice were randomly divided into experimental group (160 g/kg) and blank group (distilled water). The rats were administered orally for 2 weeks, and the acute toxicity of total flavonoids of Rhodiola rosea was observed. Results Compared with the model group, the total flavonoids of Rhodiola rosea had a significantly effect to decrease ear edema induced by xylene (P<0.05, P<0.01, P<0.01) and the inhibitory rates of the low, medium and high-dose groups were 25.75%, 38.87% and 61.13%, respectively. Granuloma induced by cotton ball were significantly inhibited (P<0.05, P<0.01, P<0.01) and the inhibitory rates of the low, medium and high-dose groups were 42.06%, 53.89% and 54.15%, respectively. The low, medium and high-dose groups could obviously decrease the concentrations of COX-2 (P < 0.01) and MAPK (P<0.05, P<0.01, P<0.01), and the medium and high-dose groups could obviously decrease the concentrations of TNF-α and IL-6 (P<0.05, P<0.01). Conclusion The total flavonoids of Rhodiola rosea has certain anti-acute and chronic inflammatory effects, and is safe and non-toxic.