2种检测方法在巧克力中沙门氏菌检验能力验证中的应用

Application of two detection methods in capability verification of Salmonella test in chocolate

中文关键词:  沙门氏菌  能力验证  检测方法

英文关键词:Salmonella  capability verification  detection methods

中文摘要:

      目的 比较国标法(生化试剂鉴定法和全自动细菌鉴定仪法)、实时荧光PCR法在能力验证项目巧克力中沙门氏菌检验中的优缺点。方法 分析2种方法的不同检测原理, 比较生化试剂鉴定法、BD Phoenix-100全自动细菌鉴定法、实时荧光PCR法在细菌鉴定应用中的优缺点。结果 3个能力验证样品检出1阳性2阴性, 2种检测方法结果一致, 3个样品实验均获得满意的结果。结论 国标的常规培养法与仪器检测方法相结合的方式, 有助于提高结果的准确性; 参加能力验证工作有助于提高实验室的检测能力。

英文摘要:

      Objective To compare the advantages and disadvantages of the national standard method (biochemical reagent identification method and automatic bacterial identification method) and real-time fluorescent PCR method in the Salmonella test in the ability verification project of chocolate. Methods The different detection principles of the two methods were analyzed to compare the advantages and disadvantages of biochemical reagent identification method, BD Phoenix-100 automatic bacterial identification method and real-time fluorescence PCR method in the application of bacterial identification. Results Totally 3 proficiency test samples were detected for 1 positive and 2 negative, and the results of the two test methods were consistent. Satisfactory results were obtained in all three sample tests. Conclusion The combination of national standard methods and instrumental detection methods helps to improve the accuracy of the results. Participating in proficiency testing helps improve laboratory testing capabilities.