高效液相色谱-串联质谱法测定花生中的黄曲霉毒素B1

Determination of aflatoxin B1 in peanut by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  黄曲霉毒素B1  花生

英文关键词:high performance liquid chromatography-tandem mass spectrometry  aflatoxin B1  peanut

中文摘要:

      目的 建立高效液相色谱-串联质谱法测定花生中黄曲霉毒素B1含量的分析方法。方法 样品经乙腈水溶液提取, 三氯甲烷反提取后, 正己烷净化, 以水(D)和乙腈(A)作为流动相进行梯度洗脱, 质谱采用多离子检测模式对黄曲霉毒素B1的定量离子和定性离子进行检测。结果 在0.5~10 ng/mL范围内线性良好(线性方程: Y=8.57×103X+312, r>0.999), 黄曲霉毒素B1在1、5、10 μg/kg 3个水平上进行加标回收试验, 平均回收率为93.2%~100.2%, 相对标准偏差为2.8%~6.9%, 方法检出限为0.1 μg/kg。结论 该方法经济、快速、准确, 适合测定花生中黄曲霉毒素B1的含量。

英文摘要:

      Objective To establish a method for the determination of aflatoxin B1 in peanut by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The samples were extracted by acetonitrile aqueous solution, reversed chloroform extraction and n-hexane purification, and eluted with water (D) and acetonitrile (A) as mobile phase. Multiple detection mode (MRM) was used to detect quantitative and qualitative ions of aflatoxin B1 by mass spectrometry. Results Within the range of 0.5-10 ng/mL, the linearity was good (linear equation: Y=8.57×103X+312, r>0.999). The average recovery rate was 93.2%-100.2%, and relative standard deviation (RSD) was 2.8%-6.9% with 3 spiked concentrations of 1, 5 and 10 μg/kg. The limit of detection was 0.1 μg/kg. Conclusion This method is economical, rapid and accurate, which is suitable for the determination of aflatoxin B1 in peanut.