高效液相色谱-蒸发光散射法同时测定山银花提取物中5种皂苷的含量

Simultaneous determination of 5 kinds of saponins in Lonicera hypoglauca extract by high performance liquid chromatography-evaporative light scattering

中文关键词:  高效液相色谱-蒸发光散射法  山银花提取物  皂苷  含量测定

英文关键词:high performance liquid chromatography-evaporative light scattering  Lonicera hypoglauca extract  saponins  content determination

中文摘要:

      目的 建立高效液相色谱-蒸发光散射法(high performance liquid chromatography-evaporative light scattering, HPLC-ELSD)同时测定山银花提取物中5种皂苷的含量的分析方法。方法 采用Agilent ZORBAX SB-C18(250 mm×4.6 mm, 5 μm), 以乙腈-0.1%甲酸溶液作为流动相进行梯度洗脱, 流速为0.8 mL/min, 柱温为30 ℃, 漂移管温度为60 ℃。结果 5种皂苷(灰毡毛忍冬皂苷乙、灰毡毛忍冬皂苷甲、川续断皂苷乙、灰毡毛忍冬次皂苷乙和灰毡毛忍冬次皂苷甲)在一定范围内线性关系良好(r为0.9989~0.9999), 回收率为95.74%~97.02%, 相对标准偏差(relative standard deviation, RSD)为0.9%~1.4% (n=6)。结论 本法简便、可靠、准确, 适用于山银花提取物的质量控制。

英文摘要:

      Objective To establish a method for simultaneous determination of 5 kinds of saponins in Lonicera hypoglauca extract by high performance liquid chromatography-evaporative light scattering (HPLC-ELSD). Methods Agilent ZORBAX SB-C18 (250 mm×4.6 mm, 5 μm) was used, and gradient elution was conducted with acetonitrile-0.1% formic acid as mobile phase for determination. Flow rate was 0.8 mL/min, column temperature was 30 ℃, and drift tube temperature was 60 ℃. Results The 5 kinds of saponins (macranthoidin A, macranthoidin B, dipsacoside B, macranthoside A and macranthoside B) had a good linear correlation in certain range (r were 0.9989-0.9999). The average recoveries were 95.74%-97.02%, and relative standard deviations (RSD) were 0.9%-1.4% (n=6). Conclusion This method is simple, reliable, accurate, which is suitable for the quality control for Lonicera hypoglauca extract.