玉米油中玉米赤霉烯酮胶体金快速检测条法和高效液相色谱检测法的比较

Comparison of rapid determination of zearalenone in corn oil by colloidal gold strip method and high performance liquid chromatography

中文关键词:  胶体金,高效液相色谱,玉米赤霉烯酮,成对样本t-检验

英文关键词:colloidal  gold, high  performance liquid  chromatography, zearalenone, paired  sample t-test

中文摘要:

      目的 建立胶体金快速检测条法测定玉米油中玉米赤霉烯酮含量的分析方法, 并与高效液相色谱法作比对。方法 分别运用胶体金快速检测条法和免疫亲和层析净化高效液相色谱检测法对14个玉米油中玉米赤霉烯酮的含量进行检测。结果 对实验结果进行成对样本t-检验, 发现2种实验方法所得出的结果在95%的置信水平上无显著差异。同时运用胶体金快速检测条法对每个样品进行6次重复实验, 分析其变异系数范围在2.9%~6.3%, 说明此种方法的重复性较好。结论 胶体金检测条法可以作为玉米油中玉米赤霉烯酮的快速检测方法。

英文摘要:

      Objective To establish a method for the determination of zearalenone in corn oil by colloidal gold strip method, and to compare with high performance liquid chromatography (HPLC). Methods The zearalenone content in 14 corn oil was detected by the methods of colloidal gold test strip and immunoaffinity chromatography purification HPLC, respectively. Results The results of the paired sample t-test showed that there was no significant difference between the 2 methods in the 95% confidence level. At the same time, the colloidal gold test strip was used to conduct 6 repeated experiments for each sample, the coefficient of variation were 2.9%-6.3%, indicating that the method has good repeatability. Conclusion The colloidal gold test strip can be used as a rapid method for the detection of zearalenone in corn oil.