吉林地区食源性沙门氏菌状况研究及快速检测

Study and Rapid Detection of Food-borne Salmonella in Jilin

中文关键词:  食源性疾病，食源性沙门氏菌，上转发光免疫层析，快速检测

英文关键词:foodborne diseases, foodborne salmonella, up-forward immunochromatography, rapid detection

中文摘要:

      目的 分析吉林市哨点医院食源性沙门氏菌监测病例，为防控食源性疾病爆发提供参考依据；为缩短检出时间，利用上转发光免疫层析技术对食源性沙门氏菌进行检测，并对其检测性能进行评价；方法 收集吉林地区哨点医院食源性沙门氏菌病例的监测信息进行研究分析；针对哨点医院细菌分离培养检出的食源性沙门氏菌，采用上转发光免疫层析方法进行检测； 结果 吉林地区食源性沙门氏菌在幼儿人群组检出率最高，疑似暴露食物主要是水果类及其制品、肉与肉制品和混合食品等三类食品，疑似暴露食物以散装最多，疑似暴露食物购买地点主要是市场，检出的食源性沙门氏菌主要是肠炎沙门氏菌和鼠伤寒沙门氏菌；对照细菌分离培养，上转发光免疫层析检测结果与其高度一致（符合率达100%）。结论 加强吉林市食品安全卫生监督，降低食源性疾病发生；上转发光免疫层析可满足食源性沙门氏菌菌株的快速检测。

英文摘要:

      Objective To analyze the surveillance cases of foodborne Salmonella in sentinel hospitals of Jilin City and provide reference for the prevention and control of outbreaks of foodborne diseases; To shorten the detection time, the detection performance of foodborne Salmonella was evaluated by up-forward photoimmunochromatography; Methods The surveillance information of foodborne salmonella cases in sentinel hospitals of Jilin Province was collected. The food-borne Salmonella detected by bacterial isolation and culture in sentinel hospitals were detected by up-forward photoimmunochromatography. Results The detection rate of food-borne Salmonella in infant population was the highest in Jilin area. The suspected food exposed was mainly fruit and its products, meat and meat products and mixed food, and the suspected food exposed in bulk was the most. Suspected exposure to food was mainly in the market, and food-borne Salmonella was detected mainly by Salmonella enteritidis and Salmonella typhimurium. Compared with bacterial isolation and culture, the results of UP-ELISA were highly consistent (coincidence rate was 100%). ConclusionStrengthening food safety and hygiene supervision in Jilin city can reduce the occurrence of foodborne diseases, and up-repeater light immunochromatography can satisfy the rapid detection of foodborne Salmonella strains.