黑咖啡提取物对人皮肤成纤维细胞胶原蛋白及衰老相关基因的影响

Effects of black coffee extract on collagen and aging related genes in human skin fibroblasts cellCui Liwen1,2,Zhang Jialing1

中文关键词:  黑咖啡  人皮肤成纤维细胞  Ⅰ型胶原蛋白  基质金属蛋白酶-1

英文关键词:black coffee  human skin fibroblasts  type I collagen  matrix metalloproteinase-1

中文摘要:

      目的 研究黑咖啡提取物对人皮肤成纤维细胞(HSF)胶原蛋白合成及衰老相关基因的影响。方法 体外培养HSF细胞,通过CCK-8法筛选黑咖啡提取物安全剂量；实验分为样品组和空白对照组,用ELISA技术检测黑咖啡提取物对细胞中Ⅰ型胶原蛋白(collagen Ⅰ,COI Ⅰ)和基质金属蛋白酶-1(MMP-1)含量的影响,用RT-PCR技术检测黑咖啡提取物作用后细胞中COI Ⅰ和MMP-1 mRNA的表达水平。结果 与空白对照组比较,黑咖啡提取物可抑制HFF-1细胞中MMP-1合成(P<0.05),促进COI Ⅰ合成(P<0.05)；使COI Ⅰ mRNA表达水平升高(P<0.01),而MMP-1 mRNA表达水平降低(P<0.01)。结论 黑咖啡提取物通过促进CoI I和抑制MMP-1表达,对胶原蛋白代谢具有改善和调节作用,可延缓皮肤衰老。

英文摘要:

      Objective To research the effects of black coffee extract on collagen synthesis of human dermal fibroblasts (HSF)cell and the aging-related genes. Methods Cultured HSF cells in vitro, and screening safe dose of black coffee extract by CCK-8 method . The experiment was divided into experimental group and control group. The effect of black coffee extract on the content of type I collagen(COI I) and matrix metalloproteinase-1(MMP-1)in HSF cells was detected by ELISA methed. The expression in mRNA levels of COI I and MMP-1 about black coffee extract were detected through RT-PCR technology.Result Compared with the control group, black coffee extract could inhibit the synthesis of MMP-1 in HSF cells (P<0.05), promote the synthesis of COI I (P<0.05), and increase the expression of COI I mRNA (P<0.01). And the expression level of MMP-1 mRNA was decreased (P<0.01). Conclusion Black coffee extract can improve and regulate collagen metabolism by promoting CoI I and inhibiting MMP-1 expression, and has the effect of inhibiting skin aging.